Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA106427 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:lncRNA expression in C2C12 myoblasts and myotubes
| PRJNA394686 | ENA
Project description:This analysis compares various timepoints from Day -1, 50% confluency myoblasts to Day 5 post differentiation myotubes. Consecutive timepoints and myoblasts vs. myotubes are compared. Keywords: time course http://www.biodatamining.org/content/1/1/4
2008-05-13 | GSE11415 | GEO
Project description:In this study, Affymetrix MG_U74Av2 and MG_U74Cv2 GeneChips were used to analyze gene expression over a 12 day time course in C2C12 myoblasts induced to differentiate in vitro. Triplicate cultures were studied during cell proliferation (days -2 and -1), at cell cycle withdrawal (day 0) and during myogenic fusion and maturation of multinucleated myotubes (days 2, 4, 6, 8, 10). The manuscript associated to this work is Tomczak et al., FASEB J., 2003 Dec 19. Additionally, the supplementary data can be found on our homepage at http://www.chb-genomics.org/beggslab/ Keywords = C2C12 myoblasts Keywords = myogenesis Keywords: time-course
2004-01-24 | GSE990 | GEO
Project description:In this study, Affymetrix MG_U74Av2 and MG_U74Cv2 GeneChips were used to analyze gene expression over a 12 day time course in C2C12 myoblasts induced to differentiate in vitro. Triplicate cultures were studied during cell proliferation (days -2 and -1), at cell cycle withdrawal (day 0) and during myogenic fusion and maturation of multinucleated myotubes (days 2, 4, 6, 8, 10). The manuscript associated to this work is Tomczak et al., FASEB J., 2003 Dec 19. Additionally, the supplementary data can be found on our homepage at http://www.chb-genomics.org/beggslab/ Keywords = C2C12 myoblasts Keywords = myogenesis Keywords: time-course
2004-01-24 | GSE989 | GEO
Project description:Transcriptome changes during the differentiation of myoblasts into myotubes
| PRJNA328159 | ENA
Project description:This study aimed to interrogate the interrelationship between 3D genome organization and global gene expression during muscle development using a mouse C2C12 cell line as an in vitro model. The C2C12 cell line is a well-established and extensively studied in vitro model derived from serial passage of myoblasts cultured from the thigh muscle of C3H mice after a crush injury. C2C12 cells divide when mitogens are present in the culture medium and spontaneously differentiate into muscle-like multinucleated (myotubes) cells if the medium is depleted of mitogens (i.e. serum; (Bischoff 1986)). C2C12 cells were either harvested as: 1) proliferating myoblasts (Myoblasts); 2) myotubes that were not treated with AraC (as such these myotubes contained myoblasts) - Myotubes(Day3); or 3) myotubes which were treated with AraC (myoblasts were largely depleted from these myotube cultures; Myotubes(Day7+AraC).
2017-04-07 | GSE84158 | GEO
Project description:In this study, the C2C12 cell line, a model used to study myogenesis and regeneration, was allowed to differentiate from myoblast precursor cells to myotubes. Cells were harvested at 3 different timepoints to perform ChIP-on-Chip of Six1, which is a key muscle regulator. We identified global loci bound by Six1 during skeletal myoblast differentiation. C2C12 Myoblasts were allowed to differentiate into myotubes. Cells at three timepoints were harvested for ChIP-on-Chip, including myoblasts stage, 24h after differentiation and myotubes (96h after differentiation). Myotubes were detached from the undifferentiated myoblast reserve cells using diluted trypsin. 3 independent biological replicates were used for each time point experiment. A microarray set counts 3 arrays (Custom Arrays A, B and C) for a total of approximately 2.9 million probes.
2010-07-05 | E-GEOD-20150 | biostudies-arrayexpress
Project description:We sought to determine the effects of over-expression of Gli1 on gene expression in C2C12 myotube cultures. C2C12 myoblasts were induced to differentiate for 4 days. At that time, when >80% of nuclei were incorporated into multi-nucleated syncitial myotubes, we infected the cultures with recombinant adenovirus expressing GFP alone or GFP and a full length human Gli1. Media was changed 12 hours later. Cultures were lysed 60 hours after the initial infection. Gli1 over-expression induces de-differentiation of myotubes and proliferation of myoblasts.
2013-12-01 | GSE37702 | GEO
Project description:Microarray time-course of mouse myotubes transduced with the transcriptional co-activator PGC-1alpha, which is known to induce mitochondrial biogenesis in muscle cells. Experiment Overall Design: Cultured mouse myoblasts (C2C12 cells) were differentiated into myotubes and on day 3 were infected with an adenovirus expressing either green fluorescent protein (GFP) or PGC-1alpha. Gene expression was measured at three time points (days 0, 1, 2, 3) in biological triplicate.
2007-12-11 | E-GEOD-4330 | biostudies-arrayexpress
Project description:We sought to determine the effects of over-expression of Gli1 on gene expression in C2C12 myotube cultures. C2C12 myoblasts were induced to differentiate for 4 days. At that time, when >80% of nuclei were incorporated into multi-nucleated syncitial myotubes, we infected the cultures with recombinant adenovirus expressing GFP alone or GFP and a full length human Gli1. Media was changed 12 hours later. Cultures were lysed 60 hours after the initial infection. Gli1 over-expression induces de-differentiation of myotubes and proliferation of myoblasts. Results provide insight into the molecular basis of SHH signaling on skeletal muscle cells.
2013-12-01 | E-GEOD-37702 | biostudies-arrayexpress