Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA107089 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Polycomb group proteins form two main complexes, PRC2 and PRC1, which generally coregulate their target genes. Here, we show that PRC1 components act as neoplastic tumor suppressors independently of PRC2 function. By mapping the distribution of PRC1 components and the histone H3K27me3 mark, we identify genes that acquire PRC1 and H3K27me3 in larvae, and a much larger set of genes bound by PRC1 in the absence of H3K27me3 in larval tissues. These genes massively outnumber canonical targets and they are preeminently involved in the regulation of cell proliferation, signaling and polarity. Mutation in PRC1 components specifically deregulates this set of genes, whereas canonical targets are derepressed in both PRC1 and PRC2 mutants. In human ES cells, PRC1 components colocalize with H3K27me3 like in Drosophila embryos, whereas they are selectively recruited to a large set of proliferation and signaling-associated genes in differentiated cell types, showing that the redeployment of PRC1 components during development is evolutionarily conserved. Comparative study of Polycomb group proteins and histon marks during development with ChIP-Seq and RNA-Seq data in eye discs and wing discs (L3 stage) in Drosophila
2016-07-18 | E-GEOD-74080 | biostudies-arrayexpress
Project description:Transcriptomes of Drosophila melanogaster eye-antennal imaginal discs at three sequential larval stages: late 2nd instar (72h after egg laying (AEL)), mid 3rd instar (96h AEL) and late 3rd instar (120h AEL).
2018-01-08 | GSE94915 | GEO
Project description:Comparative transcriptomic analysis of Drosophila melanogaster third-instar larval antennal discs and adult antennae from wild-type animals and amos mutant animals.
2021-12-13 | GSE190696 | GEO
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. This Series contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: PolII at 12 different time-points of Drosophila development. Note: 8wg16 is the name of PolII antibody for set2 datasets. Current Dataset: [GSM418514..GSM418522]: ChIP-chip of PolII in Drosophila pupae [GSM418549..GSM418557]: ChIP-chip of 8wg16 in Drosophila adult female [GSM418567..GSM418575]: ChIP-chip of 8wg16 in Drosophila embryos at 0-4 hours of development [GSM418580..GSM418588]: ChIP-chip of 8wg16 in Drosophila embryos at 4-8 hours of development [GSM418593..GSM418601]: ChIP-chip of 8wg16 in Drosophila L3 larvae [GSM442398..GSM442406]: ChIP-chip of PolII in L2 [GSM442407..GSM442415]: ChIP-chip of PolII in L3 [GSM442416..GSM442424]: ChIP-chip of 8wg16 in Drosophila embryos at 4-8 hours of development - Set2 [GSM443110..GSM443118]: ChIP-chip of 8wg16 in Drosophila embryos at 16-20 hours of development For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
2009-06-24 | GSE16702 | GEO
Project description:PSC overexpression can cause phenotypes specifically in an rbf1 mutant background, likely due to a sensitization to PSC-induced phenotypes. The goal of this study is to understand the interaction between rbf1 hypomorphic mutation and the overexpression of Polycomb group gene Posterior sex combs. We used Drosophila larval eye imaginal discs that were mutant for rbf1 or overexpressing PSC and compared these to control larval eye discs to assess changes in gene expression. We identified a common set of genes that are deregulated when rbf1 is mutated or when PSC is overexpressed. RNA was extracted from eye imaginal discs dissected from third instar Drosophila larvae. Samples were amplified and hybridized to Affymetrix Drosophila Genome 2.0 Array. To better understand the effects of rbf1 mutation and PSC overexpression, we compared the gene expression of rbf1 mutant eye discs and eye discs overexpressing PSC to control eye discs.
2014-09-30 | E-GEOD-38430 | biostudies-arrayexpress
Project description:We report on changes in gene expression resulting from the ecdysone-directed broad-based transcriptional regulatory hierarchy in Drosophila melanogaster leg imaginal discs by comparing expression in white1118 third-instar larvae (-18 hrs relative to pupariation) and white prepupae (0 hrs) to examine the transcriptional effects of the late larval ecdysone pulse, as well comparing expression in white1118 and broad5 white prepupal leg imaginal discs to examine the transcriptional effects of the early ecdysone responder, broad. Using this approach, we were able to identify critical leg disc elongation genes that are differentially regulated in broad mutants
2019-11-13 | GSE140248 | GEO
Project description:Aim: Su(H) chromatin occupancy profiling by ChIP on larval wing imaginal discs of Drosophila melanogaster to study the cooperation between Notch activation and loss of epithelial polarity (scrib mutation) during neoplastic growth. Results: The combination of Notch activation and scribble mutation (NS) does not lead to a general redeployment of Su(H) binding as compared to individual conditions (Notch only (N), and scrib mutation only (S))
2021-10-08 | GSE185490 | GEO
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: H3K27Ac at 12 different time-points of Drosophila development. Current Dataset: [GSM386179..GSM386187]: ChIP-chip of H3K27AC in Drosophila embryos at 0-4 hours of development [GSM386216..GSM386224]: ChIP-chip of H3K27AC in Drosophila embryos at 4-8 hours of development [GSM386334..GSM386342]: ChIP-chip of H3K27AC in Drosophila embryos at 0-4 hours of development (second dataset) [GSM418501..GSM418509]: ChIP-chip of H3K27AC in Adult male [GSM441405..GSM441410]: ChIP-chip of H3K27AC in Drosophila embryos at 8-12 hours of development [GSM441411..GSM441419]: ChIP-chip of H3K27AC in Drosophila embryos at 12-16 hours of development [GSM441420..GSM441436]: ChIP-chip of H3K27AC in Drosophila embryos at 16-20 hours of development [GSM441437..GSM441445]: ChIP-chip of H3K27AC in Drosophila embryos at 20-24 hours of development [GSM441446..GSM441931]: ChIP-chip of H3K27AC in L1 [GSM441933..GSM441941]: ChIP-chip of H3K27AC in L2 [GSM441943..GSM441951]: ChIP-chip of H3K27AC in L3 [GSM441984..GSM441992]: ChIP-chip of H3K27AC in Adult male [GSM441995..GSM442003]: ChIP-chip of H3K27AC in Pupae For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
2009-03-28 | GSE15430 | GEO
Project description:Two pcm null alleles (pcm14 and pcm15) were sequenced alongside respective wild-type controls (wild-type 1 and wild-type 2). Each genotype was replicated 3 times. Read depth was 12-17 million reads per replicate. Total RNA was prepared from Drosophila L3 larval wing imaginal discs.
2016-07-03 | E-MTAB-3789 | biostudies-arrayexpress
Project description:The goal of this study was to examine RNA expression levels in the Drosophila larval eye and antennal discs and determine whether higher levels of transcription were correlated with the ability of transgenes to drive pairing with their homologous endogenous loci between chromosomes. Additionally, RNA expression levels were compared between the eye and antennal discs to determine whether increased insulator protein expression contributed to increased pairing in the eye disc.
2019-08-21 | GSE136063 | GEO