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Omics score: 0

Homo sapiens

ABSTRACT: Time course of NHBE cells exposed to whole cigarette smoke

OTHER RELATED OMICS DATASETS IN: E-GEOD-10718

PROVIDER: PRJNA107573 | ENA |

REPOSITORIES: ENA

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Homo sapiens

Project description:Time course of NHBE cells exposed to whole cigarette smoke (full flavor)

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Project description:NHBE exposed to cigarette smoke

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Time course of NHBE cells exposed to whole cigarette smoke (full flavor)

Project description:Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke (CS) from a typical "full flavor" American brand of cigarettes in order to develop a better understanding of the genomic impact of tobacco exposure, which can ultimately define biomarkers that discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with CS for 15 minutes and alterations to the transcriptome assessed at 1,2,4 and 24 hours post-CS-exposure using high-density oligonucleotide microarrays. Keywords: Time course

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Transcription profiling of human NHBE cells exposed TO NORMAL human bronchial epithelial whole cigarette smoke FROM light AND standard brands

Project description:Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke from a reference cigarette (2R4F, University of Kentucky) and a typical American brand of "light" cigarettes ("Lights") in order to develop a better understanding of the genomic impact of tobacco exposure, which can ultimately define biomarkers that discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with whole cigarette smoke for 15 minutes and alterations to the transcriptome assessed at 2, 4, 8 and 24 hours post-exposure using high-density oligonucleotide microarrays. Experiment Overall Design: 4 replicate Petri dishes of cells were exposed in a custom-built smoke exposure chamber. Cigarettes were smoked as per FTC protocols, and the smoke diluted such that the cells were at least 50% viable as compared to mock (air)-exposed controls after 24h. RNA from each replicate dish was analyzed using a separate array. Four replicates of an incubator (untreated with either smoke or air) are included also.

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Project description:normal human bronchial epithelial cultures from two cultures in parallel exposed to cigarette smoke (CS) or air (mock) at timepoints 4 hours and 24 hours. Keywords = cigarette smoke Keywords = microarray Keywords = bronchial cell Keywords = tobacco Keywords: time-course

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Transcription profiling of human normal human bronchial epithelial (NHBE) cells exposed to whole cigarette smoke over a time series to study the impact of tobacco exposure

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Effect of cigarette smoke on normal human bronchial epithelial cells, and S9 toxicity

Project description:Temporal analysis of the effect of cigarette smoke on normal human bronchial epithelial cells (NHBE), and S9 toxicity. Keywords: other

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Effect of cigarette smoke on normal human bronchial epithelial cells, and S9 toxicity

Project description:Temporal analysis of the effect of cigarette smoke on normal human bronchial epithelial cells (NHBE), and S9 toxicity.

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