Project description:RNA-seq experiment comparing the transcriptomes of Bacillus cereus G9241 WT to B. cereus G9241 ∆pBCXO1 when cultured both 37 and 25 degree celsius. B. cereus G9241 is a B. cereus sensu stricto strain that was isolated from a welder with and anthrax-like illness. B. cereus G9241 carries the plasmids pBCXO1 and pBC210. pBCX01 has 99.6% sequence identity to pXO1 carried by Bacillus anthracis and encodes the tripartite anthrax toxin genes and atxA, a mammalian virulence transcriptional regulator. B. cereus G9241 WT and B. cereus G9241 ∆pBCXO1 were cultured to exponential phase at either 37 or 25 degree celsius before samples were taken for RNA extraction, library prep and sequencing.
Project description:The aim of the study was to carry out a CGH study utilizing a set of 39 diverse Bacillus isolates. Thirty four B. cereus and five B. anthracis strains and isolates were chosen so as to represent different lineages based on previous characterizations, including MLEE and MLST (Helgason, Okstad et al. 2000; Helgason, Tourasse et al. 2004). They represent the spectrum of B. cereus phenotypic diversity by including soil, dairy and periodontal isolates in addition to virulent B. anthracis strains.
Project description:Interventions: experimental group:Orally take Combined Bifidobacterium, Lactobacillus, Enterococcus and Bacillus cereus Tablets;control group:Orally take placebo
Study Design: Parallel
Project description:Comparison of the Bacillus cereus with induced ComK1 (pATK31 in sample 1-3) or ComK2 (pATK32 in sample 4-6) proteins vs Bacillus cereus carrying empty plasmid (pLM5 in all samples)
Project description:Comparison of the Bacillus cereus with overexpressed Bacillus subtilis ComK (Bacillus cereus pNWcomKBsu) vs Bacillus cereus carrying empty plasmid (Bacillus cereus pNW33N) One condition design comparision of (IPTG induced overexpression construct vs IPTG induced empty plasmid) including a dye swap, 3 biological replicate
Project description:The goal of this study is the discovery of (a) meaningful phylogenomic relationships among members of this B. cereus/B. anthracis group, and (b) reliable gene-phenotype associations, e.g. recognition of links between genomic traits and the ability of certain strains to cause various forms of disease. We also tried to elucidate genome evolution aspects that may lead to the emergence of variants that are capable (or have the potential) of causing anthrax-like disease. This large-scale comparative genomics approach is unprecedented for this taxonomic group. Dr. A. Hoffmaster (CDC) provided the PFGRC with 73 B. cereus and B. anthracis isolates from the CDC culture collection. Of these, 27 were isolated from patients with severe or systemic disease; ten isolates of this group were obtained from patients (welding factory workers) with anthrax-like disease or from the environment near their workplace. Another set of 26 represented isolates from food-born illnesses. Of the 26 gastrointestinal disease isolates (GIDI), 10 were obtained from patients with diarrhea, whereas another set of 10 had been shown to harbor the emetic (vomit) toxin gene by PCR. The rest of the group consisted of 20 isolates with various phenotypes. All strains were screened for their genomic content using the B. cereus/B. anthracis species microarray.
Project description:This SuperSeries is composed of the following subset Series: GSE13711: Comparative transcriptome and phenotype analysis of acid-stressed Bacillus cereus strain ATCC 14579 GSE13729: Comparative transcriptome and phenotype analysis of acid-stressed Bacillus cereus strain ATCC 10987 Refer to individual Series
Project description:This project contributes to the proteomic comparison of Bacillus cereus wild-type and Bacillus cereus mutant devoided of the EntD protein grown in aerobiosis condition and harvested at three growth stages.
Project description:This project contributes to the intraproteomic comparison of Bacillus cereus wild-type and Bacillus cereus mutant devoided of the EntD protein grown in aerobiosis condition and harvested at the early exponential growth stage.
