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Genomic analysis of axon pruning in Drosophila mushroom body neurons

ABSTRACT: Genomic analysis of axon pruning in Drosophila mushroom body neurons

PROVIDER: PRJNA108199 | ENA |

REPOSITORIES: ENA

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Genomic analysis of axon pruning in Drosophila mushroom body neurons

Project description:Genomic analysis of axon pruning in Drosophila mushroom body neurons identifies the RNA-binding protein Boule as a negative regulator This SuperSeries is composed of the SubSeries listed below.

2008-06-01 | GSE10014 | GEO

Transcription profiling of Drosophila mushroom body neurons at the onset and early steps of axon pruning

Project description:Drosophila mushroom body (MB) Î³ neurons undergo axon pruning during metamorphosis through a process of localized degeneration of specific axon branches. Developmental axon degeneration is initiated at the onset of metamorphosis by the pre-pupal rise in the steroid hormone ecdysone. This study identifies genes that alter their expression in MB neurons at the onset and early steps of axon pruning. Experiment Overall Design: y,w animals were staged at -18, 0 and 5 hours relative to puparium formation. RNA was isolated from MB neurons labeled with mCD8::GFP driven by OK107-GAL4 by laser capture microdissection, labeled and hybridized to Affymetrix Drosophila Genome Arrays.

2008-06-18 | E-GEOD-10012 | biostudies-arrayexpress

Transcription profiling of Drosophila mushroom body neurons

Project description:Genomic analysis of axon pruning in Drosophila mushroom body neurons identifies the RNA-binding protein Boule as a negative regulator; This SuperSeries is composed of the following subset Series:; GSE10012: Timecourse: MB neurons at the onset and early steps of axon pruning; GSE10013: EcR-dependent gene expression in MB neurons at the onset of axon pruning Experiment Overall Design: Refer to individual Series

2008-06-18 | E-GEOD-10014 | biostudies-arrayexpress

Timecourse: MB neurons at the onset and early steps of axon pruning

Project description:Drosophila mushroom body (MB) γ neurons undergo axon pruning during metamorphosis through a process of localized degeneration of specific axon branches. Developmental axon degeneration is initiated at the onset of metamorphosis by the pre-pupal rise in the steroid hormone ecdysone. This study identifies genes that alter their expression in MB neurons at the onset and early steps of axon pruning. Keywords: timecourse

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EcR-dependent gene expression in MB neurons at the onset of axon pruning

Project description:This study identifies genes that show EcR-dependent gene expression in MB neurons at the onset of axon pruning. Keywords: nuclear receptor gene regulation

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Actin dependent astrocytic infiltration is a key step for axon defasciculation during remodeling

Project description:A strocytes are essential for synapse formation, maturation and plasticity, yet their function during developmental neuronal remodeling is largely unknown To identify astrocytic molecules required for axon pruning of mushroom body ( γ neurons in Drosophi la we profile d the expression of astrocytes before ( and after ( remodeling. F ocus ing on genes enriched in larval astrocytes we identified 12 genes that are required in astrocytes, for axon pruning including the F actin regulat ors Arpc1 and form3 Interestingly, perturbing astrocytic actin dynamics did not affect their gross morphology migration or TGF β secretion In contrast, actin dynamics was required for astrocyte infiltration into the axon bundle at the onset of pruning. Remarkably, decreasing axonal adhesion facilitated the infiltration of Arpc1 KD astrocytes, and suppressed the pruning defect driven by the astrocytic perturbation Together, our findings suggest that actin dependent astrocytic infiltration is a key step in axon pruning thus promoting our understanding of neuron glia interactions during remodeling

2023-02-12 | GSE190743 | GEO

Transcription profiling of Drosophila EcR-dependent gene expression in MB neurons at the onset of axon pruning

Project description:This study identifies genes that show EcR-dependent gene expression in MB neurons at the onset of axon pruning. Experiment Overall Design: A dominant negative form of the ecdysone receptor (EcRDN) was expressed in MB neurons by OK107-GAL4. Animals were staged at 0 and 5 hours relative to puparium formation. RNA was isolated from MB neurons labeled with mCD8::GFP driven by OK107-GAL4 by laser capture microdissection, labeled and hybridized to Affymetrix Drosophila Genome Arrays. These microarrays were compared to RNA isolated from y,w animals at similar time points.

2008-06-18 | E-GEOD-10013 | biostudies-arrayexpress

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Transcriptome Analysis of Drosophila Mushroom Body Neurons by Cell Type Reveals Memory-Related Changes in Gene Expression

Project description:We report the application of low cell number sequencing of identifiable Drosophila melanogaster neurons following behavior. We demonstate the feasibility of identifying the transcriptome of 5 Mushroom Body output Neurons and 2 classes of Kenyon Cells. We find these neurons display a diverse repertoire of receptors and signaling transcripts. This information alone seems to be enough to identify each class of neurons in the study. In additional we show that aversive long-term memory induces changes in gene transcript levels in a subset of these neurons. This study provides a framework for identifying neuronal classes in Drosophila melanogaster and gaining insight into the interplay between behavior and gene regulation.

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