Project description:This SuperSeries is composed of the following subset Series:; GSE10562: Induction of ERDNp63a via Tamoxifen in primary keratinocytes; GSE10563: Primary keratinocytes treated with Tamoxifen; GSE10564: Silencing of p63 (trp63) in primary keratinocytes via siRNA oligo transfection. Experiment Overall Design: Refer to individual Series
Project description:ATAC-seq profiling of Nfat5 KO and wild type macrophages derived from bone marrow (primary cells), treated or not with Lipopolysaccharide (LPS).
Project description:α-myosin heavy chain promoter controlled MerCreMer expression enables conditional, cardiomyocyte specific and tamoxifen dependent gene inactivation of floxed genes. Administration of tamoxifen has been linked to development of acute and transient cardiomyopathy. The mechanism for this is unknown. We used microarrays to sort out factors relevant for adverse effects following tamoxifen dependent gene inactivation, to develop a protocol with minimal adverse effects, and to identify the most proper control animals.
