Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. These data were compared to those obtained in the ade16ade17 vs WT data set to determine the transcriptional effects associated to AICAR/SAICAR accumulation and those due to ade16 and ade17 deletions. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or lacking AICAR and SAICAR nucleotide derivatives.
Project description:We investigated the role of Bas1p in the transcriptional response due to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a bas1 deletion context.
Project description:We investigated the role of Pho4p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a pho4 deletion context.
Project description:We investigated the role of Pho2p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a pho2 deletion context.
Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives.
Project description:We investigated the role of Snf1p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives in a snf1 deletion context.
Project description:We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain.
