Project description:miRNA expression profiling of human monocyte-derived dendritic cells (moDCs) during maturation. Immature, 4h and 16h LPS-activated moDCs were used.
Project description:miRNA expression profiling of human monocyte-derived dendritic cells (moDCs) during maturation. Immature, 4h and 16h LPS-activated moDCs were used. In this study were analysed 2 biological samples of RNA extracted from 4h-post LPS stimulation moDCs and 2 bfrom 16h-post LPS stimulation. The RNA from 0h-post LPS stimulation were used as reference sample. Were also performed dye-swaps of each biological sample as technical replicates.
Project description:Targeted gene expression on human monocyte derived dendritic cells to investigate the effect of our adjuvant system on the maturation and activation using the nCounter Human PanCancer Immune Profiling Panel by Nanostring.
Project description:Determination of global alterations in monocyte-derived dendritic cells in response to stimulation with lipopolysaccharide using SWATH-MS to provide insight into cellular mechanism driving dendritic cell maturation
Project description:To examine the miRNA changes that occur during macrophage and dendritic cell differentiation we used the miRCURY LNATM microRNA Array (7th Gen) to profile monocyte-derived macrophages and monocyte-derived dendritic cells. Of the 2046 probes present on the assay, we detected expression of ~400 (20%) miRNAs across all samples.
