Listeria monocytogenes EGD-e
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PROVIDER: PRJNA109977 | ENA |
REPOSITORIES: ENA
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Project description:Time course study of the mouse infection by comparing the genomic transcriptional patterns of Listeria monocytogenes EGDe grown under laboratory conditions (exponential growth phase) with that of in vivo-grown bacteria (in mouse spleens) over three days of infection. Time course study of the mouse infection by comparing the genomic transcriptional patterns of Listeria monocytogenes EGDe grown under laboratory conditions (exponential growth phase) with that of in vivo-grown bacteria (in mouse spleens) over three days of infection.
2010-06-25 | E-GEOD-13057 | biostudies-arrayexpress
Project description:Time course study of the mouse infection by comparing the genomic transcriptional patterns of Listeria monocytogenes EGDe grown under laboratory conditions (exponential growth phase) with that of in vivo-grown bacteria (in mouse spleens) over three days of infection.
2009-05-29 | GSE13057 | GEO
Project description:Comparison of Listeria monocytogenes transcripts in different strains (EGD wild-type versus EGD-e wild-type, EGD-e PrfA* versus EGD-e wild-type).
2013-08-01 | E-MTAB-1676 | biostudies-arrayexpress
Project description:Comparison of Listeria monocytogenes transcripts in different strains (EGD wild-type versus EGD-e wild-type, EGD-e PrfA* versus EGD-e wild-type).
2013-08-01 | E-MTAB-1677 | biostudies-arrayexpress
Project description:Temperature dependent Transcriptome of L. monocytogenes EGDe in minimal medium
| PRJNA221635 | ENA
Project description:The foodborne pathogen Listeria monocytogenes has the ability to develop biofilm in food-processing environment, which becomes a major concern for the food safety. PrfA, a key transcriptional activator that regulates most of the known listerial virulence gene expression, has been shown to promote L. monocytogenes biofilm formation. In this study, the whole genome microarray was used to identify differentially expressed genes associated with the putative interaction between biofilm formation and PrfA in L. monocytogenes. Comparative transcriptome analyses indicated over 21.9% of the L. monocytogenes EGDe genes (627 out of 2857 predicted) were altered in their expression in biofilm cells relative to planktonic cell populations. These genes were classed into different functional categories which cover most of the biochemical functions encountered in bacterial cells, especially involved in ion transport, DNA repair, and cell wall biosynthesis based on significant enrichment of GO terms. Among them, 185 genes were identified to be associated with PrfA and biofilm formation by comparison of the whole gene expression profiles of L. monocytogenes EGDe and its M-NM-^TprfA mutant. The expression tendency of these PrfA-associated and biofilm-specific genes were mainly opposite in M-NM-^TprfA biofilm, and most of them are involved in phage-related function, membrane bioenergetics, and cell wall. Our results indicated that L. monocytogenes biofilm formation is probably controlled by the complex regulation network involved variable genes required for the different biological pathways. This regulatory network is modified in the prfA deletion mutant in order to maintain its stable biofilm lifestyle. Gene expression of planktonic cells and biofilm cells in Listeria monocytogenes EGDe and prfA isogenic deletion strain EGDeM-NM-^TprfA with cultivated in MEM and BHI for 48 hours, were mesasued using Agilent Listeria monocytogenes customized whole-genome microarray 8x15 array. Three replicates.
2013-05-01 | E-GEOD-43052 | biostudies-arrayexpress
Project description:Analysis of σL dependent traits based on phenotypic and transcriptomic evaluation of the Listeria monocytogenes EGDe strain
| PRJNA147893 | ENA
Project description:Transcriptional profile of wild type L. monocytogenes (EGDe) and a pycA mutant strain was compared on growth in BHI. The human pathogen L. monocytogenes is a facultatively intracellular bacterium that survives and replicates in the cytosol of many mammalian cells. The listerial metabolism, especially under intracellular conditions , is still poorly understood. Recent studies analyzed the carbon metabolism of L. monocytogenes by the 13C-isotopologue perturbation method in a defined minimal medium containing [U-13C6]glucose. It was shown that these bacteria produce oxaloacetate mainly by carboxylation of pyruvate due to an incomplete tricarboxylic acid cycle. Here we report that a pycA insertion mutant defective in pyruvate carboxylase (PYC) still grows, albeit at a reduced rate, in BHI medium, but is unable to multiply in a defined minimal medium with glucose or glycerol 36 as carbon source. Transcriptional profiling was performed on the pycA mutant and the wild type strain grown in BHI to get a closer insight into the effect of the pycA mutation in Listeria monocytogenes.
2010-02-10 | GSE19014 | GEO
Project description:Comparison of transcriptome of L. monocytogenes EGDe at 24M-BM-0C and 37M-BM-0C L. monocytogenes EGDe cells used in this study were grown either at 24M-BM-0C or 37M-BM-0C to an OD600 = 0.82-0.87 and the transcriptome of these two conditions was compared Six biologically independent cultures of each at 24M-BM-0C and 37M-BM-0C grown L. monocytogenes EGDe were used for total RNA isolation (six RNA sets). Three array experiments were performed with Cy5-labelled cDNA derived from at 24M-BM-0C grown Listeria and Cy3-labelled cDNA derived from at 37M-BM-0C grown cells. For the three other RNA sets a dye swap was performed, that means the three other array experiments were performed with Cy3-labelled cDNA derived form at 24M-BM-0C grown Listeria and Cy5-labelled cDNA from at 37M-BM-0C grown cells. As all oligonuceotides are spotted twice on an array, technical duplicates were performed for each experiment. The overall design results in 12 data points for the expression of each gene.
2014-05-23 | E-GEOD-51193 | biostudies-arrayexpress
Project description:Listeria monocytogenes EGD and EGD-e
| PRJNA114665 | ENA