Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA110323 | ENA |
REPOSITORIES: ENA
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Project description:Gene expression was analyzed in intestinal epithelial cells of germ-free and wildtype mice.
2009-09-04 | GSE13908 | GEO
Project description:We report that adhesion of microbes to intestinal epithelial cells is a critical cue for Th17 induction. SFB colonized in the intestine of mice can adhere to mouse small intestinal epithelial cells and induce intestinal Th17 cells. However, SFB colonized in rats cannot adhere to mouse intestinal epithelial cells and induce Th17 cells. Likewise, Citrobacter rodentium (WT) can adhere to mouse colonic epithelial cells and induce Th17 cells, but non-adherent mutant of C. rodentium (Δeae) cannot induce Th17 cells. To examine the influence of adherent bacteria on intestinal epithelial cells, we performed RNA seq. Germ free mice were orally inoculated with M-SFB or R-SFB and total RNA was isolated from small intestinal epithelial cells 1 week after inoculation. Alternatively, germ free mice were orally inoculated with C. rodentium WT or eae mutant and total RNA was isolated from colonic epithelial cells 5 days after inoculation. The gene expression of small intestinal epithelial cells isolated from small intestine of germ free mice (2 mice), mice monocolonized with M-SFB (2 mice) or R-SFB (3 mice), and colon of germ free mice (3 mice), mice monocolonized C. rodentium WT (3 mice) or eae mutant (3 mice).
2015-09-24 | E-GEOD-71734 | biostudies-arrayexpress
Project description:Germ-free versus conventional mouse terminal ileum
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Project description:We compare transcriptomic profiles of intestinal epithelial cells obtained from the small intestine of germ-free and conventionally-caged mice. Intestinal epithelial cells were harvested from the intestine of conventional or germ-free C57Bl6J mice. Directional polyA RNA-seq was performed on RNA fom cells using standard Illumina protocols. Microbiota induce decreased expression of the Clec2e gene.
2019-05-26 | GSE128362 | GEO
Project description:H3K27Ac enrichment in intestinal epithelial cells from intestine of germ-free and segmented filamentous bacteria (SFB)-monoassociated mice. Intestinal epithelial cells were harvested from the terminal ileum of germ-free or SFB mice. Chromatin immunoprecipitation was performed with anti-H3K27Ac. Sequencing was performed using the Illumina HiSeq2500. Reads were mapped to the mm10 genome using Bowtie.
2022-11-08 | GSE182628 | GEO
Project description:Intestinal epithelial cells were isolated from total small intestine of each four 28-day old conventionally raised (conv) and germ-free (GF) bred C57BL/6 mice (protocol according to: Lotz et al., J. Exp Med. 2006). Total RNA was isolated by TriZol and ist purity was examined using a Bioanalyszer. We used microarrays to comparatively detail the global gene expression in primary total isolated intestinal epithelial cells. Four biological replicates from isolated intestinal epithelial cells obtained from each 4 germ-free bred and conventionally raised mice. Colour change.
2012-05-16 | E-GEOD-35597 | biostudies-arrayexpress
Project description:Expression in intestinal epithelial cells from intestine of germ-free and segmented filamentous bacteria (SFB)-monoassociated mice during infection. Intestinal epithelial cells were harvested from the terminal ileum of germ-free or SFB mice infected with Citrobacter rodentium for 6 days. RNA was isolated using RNeasy Kit (Qiagen). Sequencing was performed using the Illumina HiSeq2500. Reads were mapped to the mm10 genome using Bowtie.
2022-11-08 | GSE182630 | GEO
Project description:We compare H3K9Ac enrichment in intestinal epithelial cells from intestine of germ-free and microbiota-replete (conventionally-housed) mice. Intestinal epithelial cells were harvested from the intestine of conventional or germ-free C57Bl6J mice. Chromatin immunoprecipitation was performed with anti-H3K9Ac. Sequencing was performed using the Illumina HiSeq2500. Reads were mapped to the mm10 genome using Bowtie. Microbiota induce loss of H3K9Ac within mulitple sites of the Clec2e gene.
2019-05-26 | GSE128369 | GEO
Project description:Expression data from Germ-Free (GF) and L.NK318.1-colonized (NK318.1) mice intestinal epithelial cells and Peyer's patches cells
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Project description:Gene expression was analyzed in intestinal epithelial cells of germ-free and wildtype mice. Experiment Overall Design: Ileums and proximal colons of 4 C57/Bl6 control and 4 C57/BL6 germ-free mice were collected and embedded in OCT. Tissue sectioned (10 mikroM) and laser capture microscopy was performed using a Zeiss/Palm laser capture system. Total RNA was extracted from about 16000 cells of the catapulted samples using RNA microprep kit (Stratagene). These 16 000 cells derive from all 4 animals (pooled approx. 4 000 cells from each mouse). We retrieved between 50 and 150 ng total RNA. Fourty ng total RNA was expanded by two rounds of in vitro cRNA amplification and affymetrix genechip Moe403 were probed at the Swegene Resource Center Lund, Sweden. Array data analysis was performed using dCHIP2004 software.
2009-09-13 | E-GEOD-13908 | biostudies-arrayexpress