Genomics

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Genome wide analysis of gene expression of rat ES cells, rat embryonic fibroblast cells and mouse ES cells


ABSTRACT: Genome wide analysis of gene expression of rat ES cells, rat embryonic fibroblast cells and mouse ES cells

PROVIDER: PRJNA110431 | ENA |

REPOSITORIES: ENA

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Rats have important advantages over mice as an experimental system for physiological and pharmacological investigations. The lack of rat embryonic stem (ES) cells has restricted the availability of transgenic technologies to create genetic models in this species. Here, we show that rat ES cells can be efficiently derived, propagated, and genetically manipulated in the presence of small molecules that specifically inhibit GSK3, MEK, and FGF receptor tyrosine kinases. These rat ES cells express pl  ...[more]

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