Project description:We use metabolite profiles of the model plant Arabidopsis thaliana measured on an UPLC-ESI/QqTOF-MS to evaluate uni- and multivariate statistical analysis of redundant features in compound spectra. Comparison was performed between the wild-type Col-0 and the 90.32 mutant. The mutant is a transposon based activation tagged A. th. line from the TAMARA population Schneider et al. [2005]. This particular mutant has an over-expression of the AT5G55880 - AT5G55890 genetic region with unknown function.
Project description:Comparison of gene expression between wild type Arabidopsis ecotype Col-0 plants and transgenic Arabidopsis ecotype col-0 plants expressing AtMYB23SRDX. Keywords: other
Project description:Macrophomina phaseolina (Mp) is a soil-borne pathogenic fungus known to infect more than 500 plants species including important crops. Here we report the use of a novel agar plate-based pathosystem using the model plant Arabidopsis thaliana (Arabidopsis) to study plant defense reponses to Mp, specifically a comparison between wild type Col-0 and double mutant ein2/jar1 roots with and without Mp infection, at two time points, by RNA-seq.
Project description:Stomataless Arabidopsis thaliana mutants spch-3 and mute-3 are extreme dwarfs but can produce cotyledons and tiny leaves, providing a system to interrogate plant life in the absence of stomata. To this end, we compared the transcriptomes of mutant cotyledons lacking SPCH or MUTE functions, and therefore lacking stomata, with those of wild-type, stomata-bearing Col-0 accession. RNA was obtained from 21 days old cotyledons collected from 9 independent biological replicates per genotype. These were pooled in 3x3 replicates and used to hybridize Affymetrix ATH1 microarrays.
Project description:Transcriptional profiling of Arabidopsis buds comparing ahl16 mutants with wild type (Col-0 ecotype). Goal was to determine the differential expression genes between the buds of mutant and wild type.
