Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA112151 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity Keywords: time-course
2003-03-28 | GSE326 | GEO
Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity Keywords: time-course
2003-03-28 | GSE325 | GEO
Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity Keywords: time-course
2003-03-28 | GSE324 | GEO
Project description:ATAC-seq profiling of Nfat5 KO and wild type macrophages derived from bone marrow (primary cells), treated or not with Lipopolysaccharide (LPS).
2021-06-09 | GSE111476 | GEO
Project description:Purpose: The goal of this study is to identify dynamic expression profiles of epigenetic genes in BMDMs induced by LPS for a time-course stimulaiton. Methods: We profiled RNA-seq in bone marrow-derived macrophages stimulated with LPS for 0h, 4h, 8h, 12h, 24h. Results: Epigenetic genes showed distinct expression patterns under LPS stimulation in different times
2022-11-13 | GSE141473 | GEO
Project description:Bone marrow-derived macrophages, Unstimulated DMSO Bone marrow-derived macrophages, Unstimulated + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 1h DMSO Bone marrow-derived macrophages, LPS 1h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 2h DMSO Bone marrow-derived macrophages, LPS 2h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 4h DMSO Bone marrow-derived macrophages, LPS 4h + I-BET (GSK525762A) LPS (100 ng/mL) was purchased from Sigma. Bone Marrow-derived macrophages (BMDMs) were differentiated from C57BL/6 bone marrow using 5 ng/mL each of recombinant M-CSF and IL-3 (Peprotech) for 7 days as described (Jeffrey et al, Nature Immunology, 2006). 2 x10^6 BMDMs were treated with DMSO or 1 μM of I-BET for 30 minutes before the addition of LPS (100 ng/mL) for 1, 2 or 4h. Unstimulated control samples were incubated with I-BET only for 1 hour. 500 ng of total RNA from 3 independent samples per group was used to prepare biotin-labeled RNA using Ambion Illumina TotalPrep RNA Amplification Kit (Applied Biosystems) and hybridized to Illumina MouseRef-8 v2.0 expression BeadChip kits. The chips were scanned using Illumina BeadArray Reader.
2010-11-10 | GSE21764 | GEO
Project description:Bone marrow-derived macrophages, Unstimulated DMSO Bone marrow-derived macrophages, Unstimulated + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 1h DMSO Bone marrow-derived macrophages, LPS 1h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 2h DMSO Bone marrow-derived macrophages, LPS 2h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 4h DMSO Bone marrow-derived macrophages, LPS 4h + I-BET (GSK525762A) LPS (100 ng/mL) was purchased from Sigma. Bone Marrow-derived macrophages (BMDMs) were differentiated from C57BL/6 bone marrow using 5 ng/mL each of recombinant M-CSF and IL-3 (Peprotech) for 7 days as described (Jeffrey et al, Nature Immunology, 2006). 2 x10^6 BMDMs were treated with DMSO or 1 μM of I-BET for 30 minutes before the addition of LPS (100 ng/mL) for 1, 2 or 4h. Unstimulated control samples were incubated with I-BET only for 1 hour. 500 ng of total RNA from 3 independent samples per group was used to prepare biotin-labeled RNA using Ambion Illumina TotalPrep RNA Amplification Kit (Applied Biosystems) and hybridized to Illumina MouseRef-8 v2.0 expression BeadChip kits. The chips were scanned using Illumina BeadArray Reader. 3 biological replicates and 4 timepoints
2010-11-10 | E-GEOD-21764 | biostudies-arrayexpress
Project description:Purpose: The goal of this study is to identify differential expression profiles between WT and Setd2-KO in BMDMs induced by LPS for a time-course stimulaiton. Methods: We profiled RNA-seq in bone marrow-derived macrophages stimulated with LPS for 0h, 12h, 24h. Results: We find significant up and down regulated genes in Setd2-KO BMDMs under LPS stimulation.
2022-11-13 | GSE141474 | GEO