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Escherichia coli str. K-12 substr. MG1655

ABSTRACT: Characterisation of Modular Reponse of non-pathogenic E.coli K12 MG1655 Response to acid adaptation, part B

PROVIDER: PRJNA114093 | ENA |

REPOSITORIES: ENA

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Escherichia coli str. K-12 substr. MG1655

Project description:Characterisation of Modular Reponse of non-pathogenic E.coli K12 MG1655 Response to acid adaptation, part A

| PRJNA114091 | ENA

Project description:Characterisation of Modular Reponse of non-pathogenic E.coli K12 MG1655 and O157 Sakai Response to acid adaptation

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Transcriptome analysis of E.coli MG1655 under multiple growth conditions

Project description:Investigation of comprehensive information about the expression level of RNA transcripts across the entire E.coli genome in mulitple growth conditions, including log-phase; stationary phase, heat shock and nitrogen-limiting condition. A fourteen chip study using total RNA recovered from four separate culture conditions of E.coli K12 MG1655. E.coli strains were harvested at mid-exponential phase with exception of stationary phase experiments. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes spaced 25 bp apart (25-bp overlap between two probes) across the E. coli genome (NimbleGen). Experiments were conducted as three or more biological replicates (different cultures)

2009-11-03 | E-GEOD-15534 | biostudies-arrayexpress

Escherichia coli O157:H7 str. Sakai

Project description:Characterisation of Modular Reponse of non-pathogenic E.coli O157 Sakai Response to acid adaptation

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YAMAT-seq of mature tRNA pools in the bacterium Escherichia coli str. K-12 substr. MG1655 and derivatives

Project description:Mature tRNA pools were measured using an adaptation of YAMAT-seq (Shigematsu et al., 2017; doi:10.1093/nar/gkx005 ) and further described in (Ayan et al., 2020; doi:10.7554/eLife.57947) in 10 strain-medium combinations (all strains dervied from the model bacterium E. coli MG1655). The aim of the experiment was to investigate the effect of reducing tRNA gene copy number on mature tRNA pools in rich and poor media.

2022-12-02 | GSE198606 | GEO

RIL-seq analysis of MG1655 Escherichia coli experiencing long-term nitrogen starvation in Gutnick minimal media

Project description:RNA interaction by ligation and sequencing (RIL-seq) was carried out to compare the Hfq-RNA-RNA interactome of MG1655 E coli bacteria that grown in Gutnick minimal media. Wild-type and hfq::3xFLAG tagged K12 E.coli were grown in Gutnick minimal media with 3mM NH4Cl as the sole nitrogen source, and cell samples were taken during exponential growth (N+), ~20min following N-runout and induction of growth arrest (N-), 24hr following N-runout and induction of growth arrest (N-24) and 2hr following addition of NH4Cl to N-24 cells (N-24+2). All sampling was performed in triplicate for each strain and condition

2023-12-01 | E-MTAB-13403 | biostudies-arrayexpress

Transcriptome profile of Escherichia coli K12 MG1655 cells lacking selected genes

Project description:The aim of the study was to compare the transcriptome of E. coli K12 MG1655 cells lacking genes known to induce the Rtc RNA repair system, such as gor, mazF and srmB, to wild-type cells. In all cases, less than 1% of the genes were found to be differentially expressed.

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Comparison of IS5 Deletion mutant to E.coli Motile and NonMotile Isolates

Project description:These series of experiments compares the expression profile of the motility variants of E.coli MG1655 ( Motile and NonMotile isolates) to an isogenic E.coli MG1655 strain in which the IS5 upstream of flhDC has been deleted. The expression profiles of genes in the E.coli MG1655 motile isolate and E.coli MG1655 Non_Motile isolate also compared. Keywords: parallel sample

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Escherichia coli str. K-12 substr. MG1655

Project description:Transcriptome analysis of E.coli MG1655 under multiple growth conditions

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RNA sequencing of Escherichia coli K12 substr. MG1655 (MTCC 1302) treated with silver nanoparticles

Project description:The current study deals to decipher the antibacterial mechanism of lysozyme coated silver nanoparticles (L-Ag NPs) (coated with lysozyme) against a Gram negative modal organism Escherichia coli K12 (MTCC 1302). Hence, the whole transcriptome profiling of E. coli K12 was done by exposing it to the MIC75 concentration of L-Ag NPs for 5 and 30 min., by RNA sequencing (RNAseq) analysis. The obtained results were utilized to understand all the metabolic pathways, signaling and molecular functions in bacterial cells under the stress of L-Ag NPs. RNAseq showed a high number of total reads along with significant ratio of high-quality reads, which confirmed the excellent quality and quantity of the obtained RNAseq data. Controlled release of ions from the surface of L-Ag NPs allowed the bacterial cells to function normally till the accumulation of threshold amount of silver ions which triggered the action of defence system, thus, reducing the chances of resistance development in bacteria. In long term, such treatment may force the bacterial machinery to induce changes in their genome to counteract the situation and develop resistance against silver ions, similar to the well-known antibiotic resistance problem. The obtained results advocate that L-Ag NPs can be used as effective antibacterial agent.

2022-06-15 | GSE151954 | GEO

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