Project description:We isolated the glucose-inhibited root elongation1 (gir1) mutant from a genetic screen of activation T-DNA tagged seeds grown on 6% glucose agar plates. The gir1 mutant showed short swollen root phenotype at the presence of glucose and had a sever sterility when grown in soil. To better understand the molecular basis of GIR1-mediated gene expression, the Affymetrix ATH1 GeneChip was used to analyze the gene expression profiling in the gir1 mutant. The results showed that cell wall, carbohydrate, and lipid metabolic or transporter-related genes were altered expression pattern. These data suggest that GIR1 regulates root and flower growth and development through cell wall modification and, perhaps, membrane trafficking.
Project description:We isolated the glucose-inhibited root elongation1 (gir1) mutant from a genetic screen of activation T-DNA tagged seeds grown on 6% glucose agar plates. The gir1 mutant showed short swollen root phenotype at the presence of glucose and had a sever sterility when grown in soil. To better understand the molecular basis of GIR1-mediated gene expression, the Affymetrix ATH1 GeneChip was used to analyze the gene expression profiling in the gir1 mutant. The results showed that cell wall, carbohydrate, and lipid metabolic or transporter-related genes were altered expression pattern. These data suggest that GIR1 regulates root and flower growth and development through cell wall modification and, perhaps, membrane trafficking. For root microarray assay [Samples GSM402178-GSM402183], wild type and the gir1 mutant plants were vertically grown on 0% glucose agar plates for 11 days, then transferred to 4% glucose plates for another ten days. Subsequently, the root tissues were excised for GeneChip analysis. In the case of inflorescence microarray assay [Samples GSM402184-GSM402191], plants were grown in soil for 33 days. The opened and opening flowers were discarded and the remaining inflorescence was harvested for GeneChip analysis.
Project description:Transcriptional profiling of Arabidopsis wild-type (Col0) control flower buds or seedlings with corresponding mutant flower buds or seedlings is performed using Aligent's Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K).
Project description:Boron is essential for plants, and boron availability in soil is an important determinant of agricultural production. Boron availability in soil is limited at many regions in the world, including Japan. Under boron deficient conditions, leaf expansion and root elongation, apical dominance, flower development,and fruit and seed sets are inhibited. In this work, we analyzed the mRNA expression of genes containing AUGUAA motif in their 5′-UTR, which is induced by boron. We used microarrays to detail the global gene expression underlying boron deficiency in roots.
