Project description:This project aimed at identifying developmental stage specific transcript profiles for catecholaminergic neurons in embryos and early larvae of zebrafish (Danio rerio). Catecholaminergic neurons were labeled using transgenic zebrafish strains to drive expression of GFP. At stages 24, 36, 72 and 96 hrs post fertilization, embryos were dissociated and GFP expressing cells sorted by FACS. Isolated RNAs were processed using either polyA selection and libray generation or NanoCAGE. This is the first effort to determine stage specific mRNA profiles of catecholaminergic neurons in zebrafish.
Project description:We performed differential DGE analysis on RNA-seq data to determine the genes misregulated in triple mutant embryos (3etv) for etv4, etv5a, and etv5b transcription factors. 3etv mutants had defects in posterior mesoderm formation, somitogenesis, and body axis straightening, as well as other Fgf related phenotypes.
