Genomics

Dataset Information

118

Synthetic construct


ABSTRACT: RNAcompete: a method for rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins

ORGANISM(S): synthetic construct  

PROVIDER: PRJNA116807 | ENA |

REPOSITORIES: ENA

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Publications

Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.

Ray Debashish D   Kazan Hilal H   Chan Esther T ET   Peña Castillo Lourdes L   Chaudhry Sidharth S   Talukder Shaheynoor S   Blencowe Benjamin J BJ   Morris Quaid Q   Hughes Timothy R TR  

Nature biotechnology 20090628 7


Metazoan genomes encode hundreds of RNA-binding proteins (RBPs) but RNA-binding preferences for relatively few RBPs have been well defined. Current techniques for determining RNA targets, including in vitro selection and RNA co-immunoprecipitation, require significant time and labor investment. Here we introduce RNAcompete, a method for the systematic analysis of RNA binding specificities that uses a single binding reaction to determine the relative preferences of RBPs for short RNAs that contai  ...[more]

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