Project description:Transcription profiling of Brassica rapa, Brassica oleracea and Brassica napus I and II The nuclear genomes of the resynthesised B. napus lines should be identical but, as one (B. napus I) involved a cross of B. oleracea onto B. rapa, and the other (B. napus II) involved a cross of B rapa onto B. oleracea, they differ in cytoplasm, and hence contain different chloroplast and mitochondrial genomes.
Project description:Transcription profiling of Brassica rapa, Brassica oleracea and Brassica napus I and II The nuclear genomes of the resynthesised B. napus lines should be identical but, as one (B. napus I) involved a cross of B. oleracea onto B. rapa, and the other (B. napus II) involved a cross of B rapa onto B. oleracea, they differ in cytoplasm, and hence contain different chloroplast and mitochondrial genomes. Four-condition experiment, comparison of transcription profiles of the genomes. Four biological replicates were used, independently grown and harvested. One replicate per array.
Project description:Global transcriptome profiling of suceptible and tolerant lines of Brassica napus infected with Sclerotinia sclerotiorum using a petal inoculation method that mimics field conditions.
Project description:Transcription profiling by array of 10 days old Brassica rapa ssp. chinensis seedlings treated with 2mM methyl jasmonate by spraying and harvesting 48 hours past treatment
Project description:Here, we employed high-throughput sequencing to identify microRNAs in CMS and its maintainer fertile (MF) lines of Brassica juncea. We identified 197 known and 78 new candidate microRNAs during reproductive development of B. juncea. A total of 47 differentially expressed microRNAs between CMS and its maintainer fertile lines were discovered, according to their sequencing read number.
Project description:Here, we employed high-throughput sequencing to identify microRNAs in CMS and its maintainer fertile (MF) lines of Brassica juncea. We identified 197 known and 78 new candidate microRNAs during reproductive development of B. juncea. A total of 47 differentially expressed microRNAs between CMS and its maintainer fertile lines were discovered, according to their sequencing read number. Two samples from floral buds of CMS and MF lines.
Project description:The aim of the experiment was to identify the transcriptional changes between wild Brassica oleraceae lines (Winspit) and 2 cultivated lines (purple sprouting broccoli and savoy cabbage) that show different biofumigation phenotypes. Fully expanded leaves were compared from 8 week old plants.
Project description:Dominant genic male sterility (DGMS) is a valuable system for hybrid seed production and improvement of plant populations, but the molecular mechanism of DGMS is not well understood. To facilitate the analysis of mechanism underlying DGMS, we compared gene expression profiles between fertile and sterile flowers of two pair of Brassica napus isogenic lines: J10AB and J03AB which only differ in sterility-related locus and fertility restore-related locus respectively by using Arabidopsis thaliana ATH-1 microarray. Gene expression profiling revealed that 360 genes differed in expression between the two lines and the number of differently expressed genes increased with progressing developmental stages. Cluster analysis result indicates that most important gene expression changes occur from PMC (anther less than 1mm) to uninucleate stage (anther in 3mm). There are 182 differential expressed genes detected between fertile plants and sterile plants at same stage. Some important pathways related to fertility control by KEGG analysis, such as glycolysis/gluconeogenesis pathway, flavonoid biosynthesis pathway and fatty acid metabolism pathway. Another abortion usually associated with suppression of gene expression of these pathways. Experiment Overall Design: The Homozygous DGMS two-type line J10AB was compared with heterozygous two-type line J03AB at three different stages of development: tetrad stage, uninucleate stage and trinucleate stage (Flower buds <1, 1-3 and >3mm, respectively) which were confirmed by cytology observation. All these flower buds were collected by hand. For each stage, two biological repetitions and two experimental repetitions were used, resulting in four hybridizations per developmental stage. Gene expression was compared between lines at different stages of development, as well as between developmental stages of fertile plants and sterile plants of each line.
Project description:Identification of differentially expressed genes in seeds and silique walls at the seed-filling stage in Brassica napus through transcriptional profiling