Project description:af13_plp2 - plp2 paraquat - Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 10 uM Paraquat was infiltated in mesophyll of leaves with a syringe without needle. Leaf material was harvested at 0, 4 and 18 hours later. 3 plant genotypes were used (Col-0 ecotype) : - siPLP2 (RNAi-silenced) - pBIN (empty pBIN-transformed) - PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),time course 6 dye-swap - CATMA arrays
Project description:af13_plp2 - plp2 paraquat - Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 10 uM Paraquat was infiltated in mesophyll of leaves with a syringe without needle. Leaf material was harvested at 0, 4 and 18 hours later. 3 plant genotypes were used (Col-0 ecotype) : - siPLP2 (RNAi-silenced) - pBIN (empty pBIN-transformed) - PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),time course
Project description:Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 10 uM Paraquat was infiltated in mesophyll of leaves with a syringe without needle. Leaf material was harvested at 0, 4 and 18 hours later. 3 plant genotypes were used (Col-0 ecotype) : - siPLP2 (RNAi-silenced) - pBIN (empty pBIN-transformed) - PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),time course 6 dye-swap - CATMA arrays
Project description:Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 10 uM Paraquat was infiltated in mesophyll of leaves with a syringe without needle. Leaf material was harvested at 0, 4 and 18 hours later. 3 plant genotypes were used (Col-0 ecotype) : - siPLP2 (RNAi-silenced) - pBIN (empty pBIN-transformed) - PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),time course
Project description:af13_plp2 - plp2 botrytis cinerea 2 - Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 5000 spores of Botrytis were pipetted on 4 infection sites per ault leaf. Leaf material was harvested at 0 and 2 days later. 3 plant genotypes were used (Col-0 ecotype): siPLP2 (RNAi-silenced),pBIN (empty pBIN-transformed),PLP2OE (PLP2-overexpressors). 4 dye-swap - CATMA arrays
Project description:Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 2500 spores of Botrytis cinerea were pipetted on 4 infection sites per adult leaf. Leaf material was harvested at 0, 24 and 48 hours. 3 plant genotypes were used (Col-0) : PLP2- silenced (RNAi), control, PLP2-overexpressors. Keywords: normal vs rnai mutant comparison,time course,treated vs untreated comparison 5 dye-swap - CATMA arrays
