Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA117167 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We demonstrate that Prnp dosage is critical for the maintenance of neuronal homeostasis since both its absence and, more relevantly, its overexpression induce higher sensitivity to kainate (KA) damage. These data correlate with electrophysiological results in freely behaving mutant mice showing an imbalance in activity-dependent synaptic processes, as determined from input/output curves, paired-pulse facilitation, and LTP studies. Gene expression profiling showed that 129 genes involved in canonical pathways such as Ubiquitination or Neurotransmission among others were co-regulated in knockout and PrPc overexpressing mice. RT-qPCR analysis of neurotransmission-related genes confirmed GABA-A and AMPA-Kainate receptor subunit transcriptional co-regulation in both Prnp -/- and Tg20 mice. Our results demonstrate that PrPc is necessary for the proper homeostatic functioning of hippocampal circuits, because of its interactions with GABAA and AMPA-Kainate receptors. Keywords: steady state expression; adult brain tissue; hippocampus; genetic modification; transgenic gain of function mutation; targetted deletion loss of function mutation
2010-01-08 | GSE16223 | GEO
Project description:We demonstrate that Prnp dosage is critical for the maintenance of neuronal homeostasis since both its absence and, more relevantly, its overexpression induce higher sensitivity to kainate (KA) damage. These data correlate with electrophysiological results in freely behaving mutant mice showing an imbalance in activity-dependent synaptic processes, as determined from input/output curves, paired-pulse facilitation, and LTP studies. Gene expression profiling showed that 129 genes involved in canonical pathways such as Ubiquitination or Neurotransmission among others were co-regulated in knockout and PrPc overexpressing mice. RT-qPCR analysis of neurotransmission-related genes confirmed GABA-A and AMPA-Kainate receptor subunit transcriptional co-regulation in both Prnp -/- and Tg20 mice. Our results demonstrate that PrPc is necessary for the proper homeostatic functioning of hippocampal circuits, because of its interactions with GABAA and AMPA-Kainate receptors. Keywords: steady state expression; adult brain tissue; hippocampus; genetic modification; transgenic gain of function mutation; targetted deletion loss of function mutation We performed a global transcriptome analysis of three strains that differ in their Prnp gene dose using Illumina Sentrix 6 mouse v1.1 beadarrays. We analyzed mRNA expression in the hippocampi of four individual male mice from each of three strains: 1) Tg20, with a 30 exogenous copies of the Prnp gene within a Prnp -/- background that overexpresses PrPc about 6-7 times the normal wild type levels; 2) littermates lacking the transgene and therefore carrying only the homozygous knock out Prnp -/- strain which lacks expression of PrPc; and 3) their wild type counterparts carrying two copies of the intact Prnp gene and expressing normal levels of PrPc
2010-04-09 | E-GEOD-16223 | biostudies-arrayexpress
Project description:Neuronal activity regulates hippocampal miRNA expression
| PRJNA147627 | ENA
Project description:Reversible phosphorylation is a critical step in the control of cellular signaling events. The protein phosphatase 1 (PP1) regulates neuronal proteins by mediating their de-phosphorylation. PP1 selectivity and subcellular localization is conferred via association with a panel of interacting proteins. We have extensively characterized the association of PP1 with inhibitor-1, an interacting protein widely expressed in brain and heart. Inhibitor-1 deletion results in a persistently active PP1 phosphatase in vitro. Inhibitor-1 overexpression results in increased transformation of cultured cells and enhances the rate of learning and memory in mouse models through largely unknown mechanisms. Significantly, inhibitor-1 activation is cAMP-sensitive. The genes regulated by inhibitor-1/PP1 signals have not been characterized. Identification of these genes is expected to provide key insights into the role of inhibitor-1/PP1 escape from cellular homeostasis and regulation of neuronal learning and memory.,We will determine gene expression patterns in hippocampus of wild-type and inhibitor-1 knock-out mice. The results of these studies of constitutive expression will be used as a baseline for future studies aimed to determine the role cAMP-mediated gene expression in I-1 knock-out mice.,We hypothesize that the genes regulated by the inhibitor-1/PP1 holoenzyme are critical to cellular growth commitment including resistance to growth arrest and neuronal learning and memory,We will examine the role of inhibitor-1 in regulating hippocampal gene expression using total RNA isolated from whole hippocampi of inhibitor-1 knockout (I-1-/-) animals and parental C57Bl6 controls under non-stimulated conditions. Animals will be sacrificed by a standard protocol. Hippocampal tissue will be rapidly dissected from several animals, flash frozen within 1 minute of extraction, pooled, and total RNA will be processed immediately using TriZol.
2007-07-07 | E-GEOD-4040 | biostudies-arrayexpress
Project description:Transitory accumulation of myeloid-derived suppressor cells in neonates is critical for maintenance of homeostasis
| PRJNA383586 | ENA
Project description:Brain postnatal development is characterized by critical periods of experience dependent remodeling. Maturation of local circuits inhibitory neurons terminate this period of enhanced plasticity. Astroglial cells are known to influence excitatory and inhibitory synaptic transmission as well as network activity through active signaling mechanisms. Although these can be developmentally regulated, the role of astrocytes in the timing of post-natal critical period is unknown. Here we show in the visual cortex that astrocytes con-trol the maturation of inhibitory neurons and thereby closure of the critical period. We uncover a novel underlying pathway involving regulation of the extracellular matrix that allows interneurons maturation via astroglial connexin signaling. We find that timing of the critical period closure is controlled by a marked upregulation of the astroglial protein connexin 30 that inhibits expression of the matrix degrading enzyme MMP9 through the RhoA-GTPase pathway. Our results thus demonstrate that astrocytes not only influ-ence neuronal activity but are also key elements in the experience–dependent wiring of brain circuits. Therefore, astrocytes represent a new cellular partner to consider in our understanding of the post-natal shaping of neuronal activities, hence providing a new target to alleviate malfunctions associated to im-paired closure of the critical period and settling of synaptic circuits.
2022-05-12 | PXD020448 | Pride
Project description:Impairment of synaptic plasticity is involved in a range of pathological conditions such as cognitive deficits. However, how synaptic efficacy is regulated is not fully understood. Here, we report that the epigenetic factor Jade family PHD finger 2 (JADE2), which is upregulated by neuronal activities, is critically involved in the maintenance of hippocampal synaptic plasticity and cognitive functions in mice. Knockdown or genetic deletion of JADE2 in hippocampal CA1 results in impaired structural and functional synaptic plasticity, leading to memory impairment, whereas overexpression of JADE2 in CA1 neurons facilitates hippocampal-dependent learning and memory. Mechanistically, we show that JADE2 modulates synaptic functions mainly by transcriptional activation of cytoskeletal protein RAC1, and this activity is dependent on its interaction with histone acetyltransferase HBO1. Together, our findings suggest JADE2 is a critical player for experience-dependent gene regulation in controlling synaptic plasticity and cognitive functions.
2022-04-11 | GSE197460 | GEO
Project description:We report that the two adult neurogenic niches of the mammalian brain – the dentate gyrus of the hippocampal formation and the subependymal zone of the lateral ventricles - displayed differential vulnerability to increased FoxG1 dosage: high FoxG1 levels severely compromised survival and glutamatergic dentate granule neuron fate acquisition in the hippocampal neurogenic niche, but left neurogenesis of GABAergic neurons in the subependymal zone / olfactory bulb system unaffected. Comparative transcriptomic analyses revealed a significantly higher expression of the apoptosis-linked nuclear receptor Nr4a1 in FoxG1-overexpressing hippocampal neural precursors. Our results reveal differential vulnerability of neuronal subtypes to increased FoxG1 dosage and suggest that activity of a FoxG1/Nr4a1 axis contributes to such subtype-specific response.
2022-03-22 | GSE185315 | GEO
Project description:MicroRNAs (miRNAs) are important regulators of gene expression; however, their contribution to protein homeostasis remains unclear. Impaired protein homeostasis could contribute to brain aging and neurodegeneration; however, the underlying mechanisms are not well understood. Here, we show that conditional inactivation of Dicer in the adult mouse brain postnatal forebrain causes age-dependent accumulation of lipofuscin and polyubiquitinated protein aggregates. Impaired protein turnover in the cerebral cortex of Dicer conditional knockout (cKO) mice is associated with protein misfolding and endoplasmic reticulum stress. Conditional Drosha inactivation using the same Cre driver results in similar phenotypes, suggesting that the phenotypes may be due to the loss of canonical miRNAs. RNA-sequencing analysis revealed increased expression of target genes of neocortical neuron-enriched miRNAs in the Dicer cKO neocortex and dysregulated expression of genes involved in protein homeostasis maintenance. Further analysis revealed a similar gene expression profile in the hippocampal CA1 region of Dicer cKO mice as in the aging brain. Moreover, translational inhibition using anisomycin or rapamycin ameliorated the protein aggregation and neurodegeneration in Dicer-deficient brains. Thus, protein translational control by miRNAs is an essential component of the protein homeostasis network controlling neuronal survival in the adult brain. Our results show that excessive translation can produce key features of human neurodegenerative diseases, and suggest that translational suppression may be a therapeutic strategy to restore protein homeostasis and combat neurodegeneration.
2023-02-15 | GSE224423 | GEO
Project description:The recognition of synaptic partners and specification of synaptic properties are fundamental for the function of neuronal circuits. 'Terminal selector' transcription factors coordinate the expression of terminal gene batteries that specify cell type-specific properties. Moreover, pan-neuronal alternative splicing regulators have been implicated in directing neuronal differentiation. However, the cellular logic of how splicing regulators instruct specific synaptic properties remains poorly understood. Here, we combine genome-wide mapping of mRNA targets and cell type-specific loss-of-function studies to uncover the contribution of the nuclear RNA binding protein SLM2 to hippocampal synapse specification. We find that SLM2 preferentially binds and regulates alternative splicing of transcripts encoding synaptic proteins, thereby generating cell type-specific isoforms. In the absence of SLM2, cell type-specification, differentiation, and viability are unaltered and neuronal populations exhibit normal intrinsic properties. By contrast, cell type-specific loss of SLM2 results in highly selective, non-cell autonomous synaptic phenotypes, altered synaptic transmission, and associated defects in a hippocampus-dependent memory task. Thus, alternative splicing provides a critical layer of gene regulation that instructs specification of neuronal connectivity in a trans-synaptic manner.
2022-12-08 | GSE220062 | GEO