Project description:Expression profiling of liver tissue from 111 F2 female mice resulting from a cross between C57BL/6J and DBA/2J inbred mouse strains. Mice were feed a chow diet for 12 months followed by 4 months on an atherogenic high-fat, high-cholesterol diet. Parental and F2 mice were killed at 16 months of age. The livers were removed immediately and flash frozen for expression profiling. See Schadt, et al., Nature , Vol 422(6929):297-302 for details. Keywords = Genetics of Gene Expression Keywords = mouse Keywords = C57BL6 Keywords = DBA Keywords: repeat sample
Project description:Expression profiling of liver tissue from 111 F2 female mice resulting from a cross between C57BL/6J and DBA/2J inbred mouse strains. Mice were feed a chow diet for 12 months followed by 4 months on an atherogenic high-fat, high-cholesterol diet. Parental and F2 mice were killed at 16 months of age. The livers were removed immediately and flash frozen for expression profiling. See Schadt, et al., Nature , Vol 422(6929):297-302 for details. Keywords = Genetics of Gene Expression Keywords = mouse Keywords = C57BL6 Keywords = DBA
Project description:Regulation of the immune response to Salmonella enterica serovar Typhimurium (S. Typhimurium) infection is a complex process, influenced by the interaction between genetic and environmental factors. Different inbred strains of mice exhibit distinct levels of resistance to S. Typhimurium infection, ranging from susceptible (e.g., C57BL/6J) to resistant (e.g., DBA/2J) strains. However, the underlying molecular mechanisms contributing to the host response remain elusive. In this study, we present a comprehensive proteomics profiling of spleen tissues from C57BL/6J and DBA/2J strains with different doses of S. Typhimurium infection by tandem tag mass coupled with two-dimensional liquid chromatography-tandem mass spectrometry (TMT-LC/LC-MS/MS). We identified and quantified 3,986 proteins, resulting in 475 differentially expressed proteins (DEPs) between C57BL/6J and DBA/2J strains. Functional enrichment analysis unveiled that the mechanism of innate immune responses to S. Typhimurium infection could be associated with several signaling pathways, including the interferon signaling pathway. We experimentally validated the roles of interferon signaling pathway in innate immune response to S. Typhimurium infection using IFN-γ neutralization assay. We further illustrated the roles of macrophage cells and pro-inflammatory cytokines in the mechanisms underlying the resistance to S. Typhimurium using qRT-PCR. Taken together, our results provide new insights into the genetic regulation of the immune response to S. Typhimurium infection in mice and might provide potential protein targets for controlling the infection.
Project description:We hypothesize that gene expression in the aging lungs of these two strains of mice are divergent thus contributing to the disparity in the phenotypes. More specifically, (1) Aging DBA/2J mice compared to aging C57BL/6 mice are known to be accelerated in their lung physiology and morphometry; (2) C57BL/6J are known to have longer natural longevity than DBA/2J mice. In order to test these hypotheses at the gene expression level, we utilized microarray analysis to examine transcriptional differences between aging lungs of both strains of mice. Keywords: comparative expression profiling