Project description:We sequenced mRNA from 12 samples extracted from mouse prefrontal cortex tissue to generate the first prefrontal cortex-specific murine transcriptome for germ-free mice (GF), conventionally raised controls (CON) and germ-free mice that have been colonized with normal microbiota from postnatal day 21 (exGF).

2016-01-14 | GSE75816 | GEO

E-GEOD-31115

Project description:Transcriptional profiling of 53 tissue samples from germ-free and conventionally raised mice

2015-07-07 | E-GEOD-31115 | ExpressionAtlas

Transcriptional profiling of intestinal tissue samples from germ-free and conventionally raised mice which are either wild-type or Myd88-/-

Project description:Whole-transcriptome survey of gene expression differences between germ-free (GF) and conventionally raised (CONV-R) mice. To assess the role of toll-like receptor signalling, both wild-type and Myd88 -/- mice were used. Four different tissues were harvested from 20 mice (5 WT CONV-R, 5 Myd88 -/- CONV-R, 4 WT GF, 6 Myd88 -/- GF) and expression profiles were determines using the Affymetrix Mouse Gene 1.0ST platform. 77 samples remained after quality control.

2011-12-08 | E-GEOD-17438 | biostudies-arrayexpress

Amydala transcriptome changes in germ-free mice

Project description:We sequenced mRNA from 12 samples extracted from mouse amygdala tissue to generate the first amygdala-specific murine transcriptome for germ-free mice (GF), conventionally raised controls (CON) and germ-free mice that have been colonized with normal microbiota from postnatal day 21 (exGF). Equal amounts of RNA from two to three animals were pooled to yield 4 samples per group (CON, GF, and exGF). Pairwise comparisons for CONvsGF, CONvsexGF, GFvsexGF were performed using DESeq2.

2015-07-09 | E-GEOD-66828 | biostudies-arrayexpress

E-GEOD-17438

Project description:Transcriptional profiling of intestinal tissue samples from germ-free and conventionally raised mice which are either wild-type or Myd88-/-

2014-07-09 | E-GEOD-17438 | ExpressionAtlas

Microbiota-dependent gene expression in small intestinal epithelial cells (IECs)

Project description:Intestinal epithelial cells were isolated from total small intestine of each four 28-day old conventionally raised (conv) and germ-free (GF) bred C57BL/6 mice (protocol according to: Lotz et al., J. Exp Med. 2006). Total RNA was isolated by TriZol and ist purity was examined using a Bioanalyszer. We used microarrays to comparatively detail the global gene expression in primary total isolated intestinal epithelial cells. Four biological replicates from isolated intestinal epithelial cells obtained from each 4 germ-free bred and conventionally raised mice. Colour change.

2012-05-16 | E-GEOD-35597 | biostudies-arrayexpress

Transcriptional profiling of 77 tissue samples from germ-free and conventionally raised mice.

2011-12-08 | GSE17438 | GEO

Transcriptional profiling of 53 tissue samples from germ-free and conventionally raised mice

2014-01-31 | GSE31115 | GEO