Project description:HT29 cells were transfected with two different PHGR1-targeted siRNA and a scramble siRNA (control) in four copies. Global mRNA profiling was performed by hybridization to Illumina Human-6 Express BeadChips (version 2). Genes significantly up- or downregulated for both PHGR1-targeted siRNAs were identified.
Project description:The FAT1 gene was knocked down using 2 independent siRNAs, in immortalized human astrocytes and U87 and U251 glioma cell lines. A non-targeted scramble siRNA was used as a control. Scramble, FAT1 siRNA#1 and FAT1 siRNA#2 were transfected into each cell line, in duplicate, and RNA analyzed using the Affymetrix U133A 2.0 platform.
