Project description:Human mesenchymal stem cells (MSC) derived from perirenal adipose tissue (PV) of living kidney donors were cultured under various conditions, namely (1) control (medium+foetal bovine serum(FBS)) or (2) control (medium+heat-inactivated FBS); (3) with mixed-lympohocyte reactions (MLR) in transwell culture systems for 4 days; (4) with mixed-lympohocyte reactions (MLR) in transwell culture systems for 7 days; or (5)with pro-inflammatory cytokines(IFNgamma, TNFalpha and interleukin 6). We used microarrays to detail the effect of the various culture conditions on MSC.
Project description:Human mesenchymal stem cells (MSC) derived from perirenal adipose tissue (PV) of living kidney donors were cultured under various conditions, namely (1) control (medium+foetal bovine serum(FBS)) or (2) control (medium+heat-inactivated FBS); (3) with mixed-lympohocyte reactions (MLR) in transwell culture systems for 4 days; (4) with mixed-lympohocyte reactions (MLR) in transwell culture systems for 7 days; or (5)with pro-inflammatory cytokines(IFNgamma, TNFalpha and interleukin 6). We used microarrays to detail the effect of the various culture conditions on MSC. MSC were cultured under control conditions (n=4 in FBS and n=3 in heat-inactivated FBS), with MLR in transwell culture systems for 4 days (n=3) and 7 days (n=4), and with pro-inflammatory cytokines(IFNgamma, TNFalpha and interleukin 6)(n=4) and collected for gene analysis
Project description:This SuperSeries is composed of the following subset Series: GSE25068: PcG/TrxG profiling of differentially aged adipose-derived mesenchymal stem cells GSE25069: Whole-genome microarray of long-term cultured adipose derived mesenchymal stem cells from differentially-aged mice GSE25679: microRNA profiling of mesenchymal stem cells from adipose tissue of differentially aged mice Refer to individual Series
Project description:Human adipose and bone marrow-derived mesenchymal stem cells were cultured either on collagenase biomaterial (CardioCel®) or normal tissue culture plastic over 48 hours in standard culture conditions, in serum-free medium.
Project description:Regardless of its anatomical site, adipose tissue shares a common energy-storage role but exhibits distinctive properties. Exploring the cellular and molecular heterogeneity of white adipose tissue (WAT) is crucial for comprehending its function and properties. In this study, we employed Single nucleus RNA sequencing (snRNA-seq) to test five representative depots including inguinal, epididymal, mesenteric, perirenal, and pericardial adipose tissues in mice under physiological conditions. By analyzing the contents of main cell categories and gene profiles of various depots, we identified their distinctive physiological properties.
Project description:The aim of this study was to explore the gene expression and metabolites among multisite adipose-derived mesenchymal stem cells, and investigate the metabolic pathway of multisite adipose-derived mesenchymal stem cells using a multi-omics analysis. Subcutaneous adipose-derived mesenchymal stem cells (SASCs), perirenal adipose-derived mesenchymal stem cells (PASCs), and epididymal adipose-derived mesenchymal stem cells (EASCs) were isolated from Sprague Dawley rats. RNA and metabolites were extracted and sequenced using transcriptomics and metabolomics analyses, respectively. There were 720 differentially expressed genes (DEGs) in EASCs and 688 DEGs in PASCs compared with SASCs; there were 166 unique DEGs in EASCs, 134 unique DEGs in PASCs, and 554 common DEGs between EASCs and PASCs. Furthermore, there were 220 differential metabolites in EASCs, 249 differential metabolites in PASCs, 83 unique differential metabolites in EASCs, 112 unique differential metabolites in PASCs, and 137 common differential metabolites between EASCs and PASCs. The transcriptomics and metabolomics analyses identified four hub genes, one in EASCs and three in PASCs. There are functional differences among multisite adipose-derived mesenchymal stem cells that may be related to the hub genes Atac2, Rrm1, Rrm2, and Gla. The relevant signaling pathways are the Ras signaling pathway, HIF-1 signaling pathway, and the p53 signaling pathway.
Project description:Human adipose-derived mesenchymal stem cells were cultured either in hypoxia (Hx; <0.1% oxygen) or standard culture conditions (normoxia, Nx) over 48 hours in serum-free medium. Human tympanic membrane keratinocytes were cultured in standard culture conditions over 48 hours in serum-free medium.
