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Streptococcus mutans UA159 vs. spx mutant strains

ABSTRACT: Streptococcus mutans UA159 vs. spx mutant strains

PROVIDER: PRJNA122557 | ENA |

REPOSITORIES: ENA

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Streptococcus mutans UA159 control vs. Streptococcus mutans UA159 supplemented with 20 uM 4,5-dihydroxy-2,3-pentadione (DPD) at 3 different pH values

Project description:Transcriptional profiling of early logarithmic phase culture (O.D=0.2-0.3) of Streptococcus mutans UA159 comparing control of untreated Streptococcus mutans UA159 bacteria with Streptococcus mutans UA159 bacteria spplemented with 20µM synthetic DPD (pre-AI-2) which regulates gene expression via AI-2 quorum sensing system.Three compairisons were performed at pHs of 7,6 and 5.

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Streptococcus mutans UA159 vs. spx mutant strains

Project description:Transcriptional profiling to investigate the regulatory roles of SpxA and SpxB of S. mutans. RNA was extracted from four replicate samples of each strain of interest (spxA mutant, spxB mutant, spxAB double-mutant, UA159 wild type) and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cells grown to mid-log.

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Streptococcus mutans UA159 vs. fabM mutant strain (UR117), pH7 vs. pH5/5.5

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Streptococcus mutans UA159; 10 mM Glucose Versus 100 mM Glucose

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