Project description:The miRNA 310-311-312-313 cluster from D. melanogaster (referred to as Dm310s) and the homolog from D. pseudoobscura (Dp310s) were used to transform D. melanogaster. The over-expression of UAS-miR310s in the Dm310s transgenic line M1-7 and Dp310s transgenic line P4-18 was driven by GAL4 line NP5941 with native miR-310 expression pattern. We used DGRC-1 cDNA arrays to assess the effects of miR310s over-expression on the whole transcriptome.
Project description:The miRNA 310-311-312-313 cluster from D. melanogaster (referred to as Dm310s) and the homolog from D. pseudoobscura (Dp310s) were used to transform D. melanogaster. The over-expression of UAS-miR310s in two Dm310s transgenic lines (M1-7 and M1-3) and Dp310s transgenic line P4-18 was driven by GAL4 line NP5941 with native miR-310 expression pattern. We used Drosophila Tiling 1.0 F arrays to assess the effects of miR310s over-expression on the whole transcriptome.
Project description:The miRNA 310-311-312-313 cluster from D. melanogaster (referred to as Dm310s) and the homolog from D. pseudoobscura (Dp310s) were used to transform D. melanogaster. The over-expression of UAS-miR310s in the Dm310s transgenic line M1-7 and Dp310s transgenic line P4-18 was driven by GAL4 line NP5941 with native miR-310 expression pattern. We used DGRC-1 cDNA arrays to assess the effects of miR310s over-expression on the whole transcriptome. Direct two-colour design involving six arrays in three dye-swap pairs, with each pair used for the pairwise comparison of Dm310s, Dp310s and w1118 (control).
Project description:The miRNA 310-311-312-313 cluster from D. melanogaster (referred to as Dm310s) and the homolog from D. pseudoobscura (Dp310s) were used to transform D. melanogaster. The over-expression of UAS-miR310s in two Dm310s transgenic lines (M1-7 and M1-3) and Dp310s transgenic line P4-18 was driven by GAL4 line NP5941 with native miR-310 expression pattern. We used Drosophila Tiling 1.0 F arrays to assess the effects of miR310s over-expression on the whole transcriptome. We compared the transcriptional profiling of Dm310s and Dp310s overexpression in D.melanogaster using double-stranded cDNA followed by bioprime random labeling, and hybridization to Affy Drosophila tiling 1.0 F array. Third-instar progeny larvae from the crosses with maternal NP5941 and paternal UAS-miR-310 cluster transgenic lines were collected and third-instar larvae from the cross with maternal NP5941 and paternal w1118 were used as control. Three biological repeats were used for each stock. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.
Project description:Expression profiling of the dme-miR-310 cluster knockout line in D.melanogaster. The knockout line was generated in an imprecise P-element excision screen using a stock P{EP}2587 containing a P-element insertion directly upstream of the miR-310 cluster. The precise excision line of P{EP}2587 was used as the control. The expression of miR-310 cluster knockout line (imprecise deletion of P{EP}2587 insertion) were compared with the control (precise deletion of P{EP}2587 insertion) using Drosophila Tiling 1.0 F array. Each stock has three biological repeats. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.
Project description:Expression profiling of the dme-miR-310 cluster knockout line in D.melanogaster. The knockout line was generated in an imprecise P-element excision screen using a stock P{EP}2587 containing a P-element insertion directly upstream of the miR-310 cluster. The precise excision line of P{EP}2587 was used as the control.