Global studies of transcript structure and abundance in cancer cells enable the systematic discovery of aberrations that contribute to carcinogenesis, including gene fusions, alternative splice isoforms, and somatic mutations. We developed a systematic approach to characterize the spectrum of cancer-associated mRNA alterations through integration of transcriptomic and structural genomic data, and we applied this approach to generate new insights into melanoma biology. Using paired-end massively ...[more]
Project description:This SuperSeries is composed of the following subset Series: GSE17349: Expression data for melanoma short-term cultures and cell lines GSE17359: Affymetrix SNP array data for 3 melanoma short-term cultures and cell lines GSE20156: RNA-Seq of melanoma short-term cultures and cell lines Refer to individual Series
Project description:We profiled the gene expression levels from 8 melanoma short-term cultures and 1 melanoma cell line in order to compare to expression level estimates obtained by RNA-seq. Overall design: Melanoma short-term cultures and cell lines were propagated in vitro, and total RNA was extracted for microarray hybridization and sequencing. The 9 Samples report the microarray hybridization results.
| GSE17349 | GEO
Project description:Melanoma short-term cultures and cell lines: expression profiling and CNV analyses
Project description:Investigation of expression differences induced by expression of the histone methyltransferase SETDB1 in human melanoma short-term culture WM451-Lu. A six-chip study using total RNA prepared from WM451-Lu melanoma short-term cultures infected with either a lentivirus encoding GFP (control) or SETDB1. Cells were allowed to grow for 2 days post-infection.