Dataset Information


Mus musculus

ABSTRACT: DNA methylation comparison of ntTS, TS and ES cells

ORGANISM(S): Mus musculus  



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Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos.

Oda Mayumi M   Tanaka Satoshi S   Yamazaki Yukiko Y   Ohta Hiroshi H   Iwatani Misa M   Suzuki Masako M   Ohgane Jun J   Hattori Naka N   Yanagimachi Ryuzo R   Wakayama Teruhiko T   Shiota Kunio K  

Proceedings of the National Academy of Sciences of the United States of America 20090824 38

Placental abnormalities occur frequently in cloned animals. Here, we attempted to isolate trophoblast stem (TS) cells from mouse blastocysts produced by somatic cell nuclear transfer (NT) at the blastocyst stage (NT blastocysts). Despite the predicted deficiency of the trophoblast cell lineage, we succeeded in isolating cell colonies with typical morphology of TS cells and cell lines from the NT blastocysts (ntTS cell lines) with efficiency as high as that from native blastocysts. The establishe  ...[more]

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