Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA123457 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene expression profiles of renin producing cells in the adult kidney (captopril treated animals) isolated from Renin-YFP transgenic mice using FACS (GUDMAP Series ID:46).
| PRJNA182920 | ENA
Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Renin-YFP transgenic mice were utilized to isolate renin producing cells from the kidneys of Adult mice treated with captopril, which was provided in the drinking water at a concentration of 0.5mg/ml. In addition, mice were given low-salt chow. Mice were treated for two weeks before renin producing cells were isolated from the kidney by fluorescent activated cell sorting (FACS). RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.
2012-12-05 | E-GEOD-42712 | biostudies-arrayexpress
Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone Renin-GFP transgenic mice were utilized to isolate renin producing cells from the kidneys of either P0 neonatal pups or Adult mice. Renin producing cells were isolated from the kidney by fluorescent activated cell sorting (FACS). RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.
2010-07-16 | E-GEOD-17138 | biostudies-arrayexpress
Project description:Gene expression profiles of E15.5 cap mesenchyme isolated from Six2 transgenic mice using FACS. (GUDMAP Series ID: 23)
| PRJNA112835 | ENA
Project description:Gene expression profiles of E15.5 peripheral blastema isolated from Meis1-EGFP transgenic mice using FACS. (GUDMAP Series ID: 17)
| PRJNA101163 | ENA
Project description:Gene expression profiles of E15.5 endothelial cells isolated from TIE2-GFP transgenic mice using FACS. (GUDMAP Series ID:38)
| PRJNA124503 | ENA
Project description:Gene expression profiles of adult mesangial cells isolated from Meis1-EGFP transgenic mice using FACS. (GUDMAP Series ID: 37)
| PRJNA124833 | ENA
Project description:Gene expression profiles of E10.5 ureteric tip cells isolated from Hoxb7-GFP transgenic mice using FACS. (GUDMAP Series ID: 39)
| PRJNA126231 | ENA
Project description:Gene expression profiles of E10.5 ureteric trunk cells isolated from Hoxb7-GFP transgenic mice using FACS. (GUDMAP Series ID: 40)
| PRJNA127023 | ENA
Project description:Gene expression profiles of adult glomerular endothelial cells isolated from TIE2-GFP transgenic mice using FACS. (GUDMAP Series ID: 42)
| PRJNA128445 | ENA