Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA123459 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We used micro-dissection with FACS sorting techniques to isolate renal vesicle single cell types from post natal (P4) kidneys. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Kidneys are harvested from Tg(Crym-EGFP)GF82Gsat mice. Single cells are extracted from P4 renal vesicles using micro-dissection with FACS sorting techniques. A subset of these cells is analyzed individually via Fluidigm single cell analysis. The long term goal is to generate a transcriptional atlas of the developing kidney.
2014-08-01 | E-GEOD-59130 | biostudies-arrayexpress
Project description:Gene expression profiles of E15.5 cap mesenchyme isolated from Six2 transgenic mice using FACS. (GUDMAP Series ID: 23)
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Project description:We used micro-dissection with FACS sorting techniques to isolate single cells from the metanephric mesenchyme of the E11.5 developing kidney. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Kidneys are harvested from Tg(Crym-EGFP)GF82Gsat mice. Single cells are extracted from E11.5 metanephric mesenchyme using manual micro-dissection techniques. A subset of these cells is analyzed individually via Fluidigm single cell analysis. The long term goal is to generate a transcriptional atlas of the developing kidney.
2014-08-01 | E-GEOD-59129 | biostudies-arrayexpress
Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. At E18.5, pups were isolated from CRYM-BAC transgenic mice and fostered to CD-1 mice. Cap mesenchyme cells were isolated from the kidney by fluorescent activated cell sorting (FACS) on postnatal days 0, 1, 2, 3, and 4. RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.
2010-07-16 | E-GEOD-17139 | biostudies-arrayexpress
Project description:We used micro-dissection techniques and/or FACS to isolate cell types from the developing and adult kidney (E11.5 ureteric buds, E12.5, P1 and P4 cap mesenchyme, E15.5 collecting ducts, proximal tubules, ureter, Adult renal proximal tubules, podocytes, endothelial and mesangial cells). RNA-SEQ analysis was performed to determine the transcriptional profile of each cell type, identify component specific transcripts and isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Total RNA is obtained from micro-dissected and/or FACS isolated embryonic and adult kidney components. The long term goal is to generate a transcriptional atlas of developing kidney.
2015-01-27 | E-GEOD-64959 | biostudies-arrayexpress
Project description:We used micro-dissection techniques and/or FACS to isolate cell types from the developing and adult kidney (E11.5 ureteric buds, E12.5, P1 and P4 cap mesenchyme, E15.5 collecting ducts, proximal tubules, ureter, Adult renal proximal tubules, podocytes, endothelial and mesangial cells). RNA-SEQ analysis was performed to determine the transcriptional profile of each cell type, identify component specific transcripts and isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database.
2015-01-27 | GSE64959 | GEO
Project description:Gene expression profiles of E15.5 peripheral blastema isolated from Meis1-EGFP transgenic mice using FACS. (GUDMAP Series ID: 17)
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Project description:Gene expression profiles of adult mesangial cells isolated from Meis1-EGFP transgenic mice using FACS. (GUDMAP Series ID: 37)
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Project description:RNA-SEQ analysis of single cells extracted from the metanephric mesenchyme of E11.5 Crym-EGFP transgenic mice
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Project description:RNA-SEQ analysis of single cells extracted from the renal vesicles of P4 Crym-EGFP transgenic mice
| PRJNA254484 | ENA