Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA123883 | ENA |
REPOSITORIES: ENA
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Project description:This SuperSeries is composed of the following subset Series: GSE18952: Effects of IGF 1 on primary breast fibroblasts (normal and carcinoma associated) GSE18953: Effects of IGF 1 on MCF-7 breast cancer cells GSE18954: Effects of IGF 1 on CCL-171 fibroblasts Refer to individual Series
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Project description:To characterize the effects of IGF 1 on CCL171 cells, we cultured pre-starved CCL 171 fibroblasts with or without 50ng/ml IGF 1 for 24 h. We then profiled gene expression changes using Human Exonic Evidence Based Oligonucleotide (HEEBO) microarrays. After stimulation, total RNA was extracted and amplified using a modified Eberwine procedure. The amplified RNA was labeled with the fluorescent dye Cy5 and pooled with Cy3 labeled reference RNA, and then the pooled RNA was hybridized onto HEEBO microarrays. After hybridization and washing, arrays were scanned on a fluorescent microscope scanner.
2010-01-21 | GSE18954 | GEO
Project description:To characterize the effects of IGF 1 on CCL171 cells, we cultured pre-starved CCL 171 fibroblasts with or without 50ng/ml IGF 1 for 24 h. We then profiled gene expression changes using Human Exonic Evidence Based Oligonucleotide (HEEBO) microarrays. After stimulation, total RNA was extracted and amplified using a modified Eberwine procedure. The amplified RNA was labeled with the fluorescent dye Cy5 and pooled with Cy3 labeled reference RNA, and then the pooled RNA was hybridized onto HEEBO microarrays. After hybridization and washing, arrays were scanned on a fluorescent microscope scanner. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
2010-06-24 | E-GEOD-18954 | biostudies-arrayexpress
Project description:To assess the transcriptomic response of human lung fibroblast to TGF-β, human MRC5 pulmonary fibroblasts (CCL-171) were exposed to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Three independent experiments were carried out.
2019-04-11 | GSE97832 | GEO
Project description:Human MRC5 pulmonary fibroblasts (CCL-171) were exposed or not to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Two independent experiments were carried out.
2019-04-11 | GSE97833 | GEO
Project description:Effects of IGF 1 on primary breast fibroblasts (normal and carcinoma associated)
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Project description:To assess the transcriptomic response of human lung fibroblast to TGF-β, human MRC5 pulmonary fibroblasts (CCL-171) were exposed or not to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Three independent experiments were carried out.
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Project description:Effect of heterotypic interaction between breast cancer cell line MDA-MB231 and CCL-171 fibroblast
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Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
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