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Homo sapiens

ABSTRACT: Human variation in PolII and NF-KappaB binding (ChIP-seq study with NF-KappaB)

OTHER RELATED OMICS DATASETS IN: GSE19486

PROVIDER: PRJNA123953 | ENA |

REPOSITORIES: ENA

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Human variation in PolII and NF-KappaB binding (ChIP-seq study with Pol II)

Project description:Human variation in PolII and NF-KappaB binding (ChIP-seq study with Pol II)

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Human variation in PolII and NF-KappaB binding (ChIP-seq study with NF-KappaB)

Project description:We examined genome-wide variation in transcription factor binding in different individuals and a chimpanzee using chromatin immunoprecipitation followed by massively-parallel sequencing (ChIP-Seq). The binding sites of RNA Polymerase II (Pol II) as well as a key regulator of immune responses, NFkB, were mapped in ten HapMap lymphoblastoid cell lines derived from individuals of African, European, and Asian ancestry, including a parent-offspring trio. We also mapped gene expression in all ten human cell lines for two treatment conditions: a) no treatment and b) following induction by TNF-alpha. Genome-wide comparison of Pol II and NF-KappaB binding in ten individuals. ChIP-seq with NF-KappaB.

2010-03-22 | E-GEOD-19485 | biostudies-arrayexpress

Human variation in PolII and NF-KappaB binding (ChIP-seq study with Pol II)

2010-03-22 | E-GEOD-19484 | biostudies-arrayexpress

Human variation in PolII and NF-KappaB binding (RNA-seq study with TNF-alpha induced)

2010-03-22 | E-GEOD-19481 | biostudies-arrayexpress

Human variation in PolII and NF-KappaB binding (RNA-seq study uninduced by TNF-alpha)

2010-03-22 | E-GEOD-19466 | biostudies-arrayexpress

p65-HaloTag copurifying proteins in human 293T cells

Project description:In effort to develop methodology for targeted top down mass spectrometry of NF kappa B p65 from human cells, we evaluated the utility of HaloTag for purification and analysis of recombinant protein. During our study, two datasets of bottom up LC-MS/MS were generated: one from in-gel digestion of the predominant band following p65-HaloTag purification, another from in-solution digestion of all the proteins present in a p65-HaloTag purification. p65-HaloTag copurifying proteins identified in our datasets include the known interactors c-Rel, NF-kappaB p105, NF-kappaB p100, and NF-kappaB inhibitor beta. Over 100 proteins were identified by at least two peptides using a Mascot ion cut-off score of 30.

2018-10-19 | PXD001932 | Pride

RelA Nuclear factor-kappaB (NF-kB) Subunit binding Loci in Promoter Regions of PHM1-31 Myometrial Smooth Muscle Cells (Promoter)

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