Genomics

Dataset Information

235

RNA-Seq on libraries made from ERCC external RNA controls, and a mixture of D. melanogaster S2 cell mRNA and ERCC mRNAs


ABSTRACT: RNA-Seq on libraries made from ERCC external RNA controls, and a mixture of D. melanogaster S2 cell mRNA and ERCC mRNAs

PROVIDER: PRJNA125273 | ENA |

REPOSITORIES: ENA

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Publications

Synthetic spike-in standards for RNA-seq experiments.

Jiang Lichun L   Schlesinger Felix F   Davis Carrie A CA   Zhang Yu Y   Li Renhua R   Salit Marc M   Gingeras Thomas R TR   Oliver Brian B  

Genome research 20110804 9


High-throughput sequencing of cDNA (RNA-seq) is a widely deployed transcriptome profiling and annotation technique, but questions about the performance of different protocols and platforms remain. We used a newly developed pool of 96 synthetic RNAs with various lengths, and GC content covering a 2(20) concentration range as spike-in controls to measure sensitivity, accuracy, and biases in RNA-seq experiments as well as to derive standard curves for quantifying the abundance of transcripts. We ob  ...[more]

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