Project description:Transcriptional profiling in young flowers (stage 8) of Arabidopsis wild type control plants and sdg2-1 mutant is performed using Aligent’s Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K). A significant number of genes involved in gametophyte development are found differentially regulated in the sdg2-1 mutant.
Project description:Transcriptional profiling in young flowers (stage 8) of Arabidopsis wild type control plants and sdg2-1 mutant is performed using Aligentâs Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K). A significant number of genes involved in gametophyte development are found differentially regulated in the sdg2-1 mutant. Two-condition experiment, young flowers of wild type control vs. young flowers of sdg2-1 mutant. Two biological replicates: 2 control, 2 mutant, independently grown and harvested. One replicate per array.
Project description:Transcriptional profiling in open flowers of Arabidopsis wild type control plants and sdg4 mutant (SALK T-DNA line_128444). The sdg4 mutant is a Arabidopsis T-DNA mutant in which T-DNA is inserted in a SET domain protein, SDG4 (At4g30860). Expression profiling studies indicate that SDG4 might function in modulating the expression of the genes that function in the growth of pollen tubes. Keywords: epigenetic modification
Project description:au05-03_gaba - ler vs pop2-1: gaba over-accumulation effects - The analysis aims at identifying genes that are differentially regulated by the over-accumulation of GABA observed in the mutant pop2-1 in response to treatment with exogenous GABA and that may explain the singular phenotype of the mutant in this condition. Designed experiment consisted in comparison of transcriptomes of Arabidopsis thaliana Landsberg erecta ecotype and its mutant pop2-1 (impaired in GABA transaminase activity) during a kinetic of endogenous GABA accumulation. For this purpose, 10-day-old plants grown on half strength Hoagland's agar medium were transferred to agar plates supplemented with 1 mM GABA. We isolated RNA from plants treated for 0, 1 and 4 days. Treatments were made in duplicate. Keywords: gene knock-out, time course
Project description:Although two genes responsive to GABA were characterized previously in plants (Kathiresan et al 1997), a comprehensive study in reproductive and vegetative tissues has not been performed. Consequently, genes that mediate GABA response in plants remain unknown. We therefore used microarrays to survey the genome, identifying genes modulated in response to GABA in reproductive tissues. Flowers were collected as described in the protocols section for RNA extraction and hybridization on Affymetrix ATH1 Genechip microarrays. Gene expression in wild-type and pop2-1 mutant flowers containing high levels of endogenous GABA were compared.
