Project description:Analysis of proteins under the influence of the quorum sensing (QS) system in the nonpathogenic Agrobacterium tumefaciens strain 6N2, and the influence of the bacterial QS system in the proteome of the yeast Meyerozyma guilliermondii strain 6N.
Project description:Antagonistic yeasts inhibit the growth of fungal. In our previous research, Meyerozyma guilliermondii one of antagonistic yeasts exhibits antagonistic activity against Penicillium expansum. However, the mechanisms, especially the molecular mechanisms of inhibiting activity of M. guilliermondii are not clear. In this study, the transcriptome characterization of P. expansum induced by M. guilliermondii were investigated.
Project description:Meyerozyma guilliermondii, which was isolated from pears by our laboratory, M. guilliermondii had a significant effect on the induction of several defense-related genes compared to the control. It revealed the defense response mechanism of pears by transcriptomics, and investigated the function of more defense-related genes in pears.
Project description:Pathogenic Candida fungi are a leading cause of opportunistic, hospital-associated bloodstream infections with high mortality rates, typically in immunocompromised patients. Several species, including C. albicans, the most prevalent cause of infection, belong to the monophyletic CUG clade of yeasts. Much is known about the interaction of C. albicans with innate immune cells, which are crucial for controlling infection. Phagocytosis of C. albicans elicits transcriptional induction of several pathways involved in catabolism of non-glucose carbon sources that are important for virulence, termed alternative carbon metabolism. However, the response of other CUG clade species has not been characterized. In a separate dataset, we profiled transcriptional responses to primary murine bone marrow derived macrophages in six Candida species. Here we additionally profiled the response of M. guilliermondii, a yeast that is known as a cause of disseminated candidiasis as well as cutaneous infections. We find that similar to other CUG-clade Candida species, it mounts a robust alternative carbon metabolism response to phagocytosis.
