Project description:This SuperSeries is composed of the following subset Series: GSE22360: Transcriptomic adaptations to symbiotic life in cnidarians : symbiotic vs bleached Anemonia viridis sea anemones GSE22361: Endoderm- vs ectoderm-specific expression of symbiosis genes in the snakelocks sea anemone Refer to individual Series
Project description:Transcriptional comparison between symbiotic and non-symbiotic (bleached) sea anemones Anemonia viridis were analysed in several specimens. We generated an oligonucleotide microarray (2000 selected features), which is to date the only available oligonucleotide array for symbiotic cnidarians. We were able to identify a subset of genes clearly involved in symbiosis.
Project description:Transcriptional comparison between symbiotic and non-symbiotic (bleached) sea anemones Anemonia viridis were analysed in several specimens. We generated an oligonucleotide microarray (2000 selected features), which is to date the only available oligonucleotide array for symbiotic cnidarians. We were able to identify a subset of genes clearly involved in symbiosis. Whole tentacle samples were prepared from 5 symbiotic and 5 bleached specimens. Hybridizations were performed against a single reference (VBl) in a dye-swap experiment.
Project description:Transcriptional comparison between endodermal and ectodermal compartment in symbiotic sea anemones Anemonia viridis were analysed in several specimens. We generated an oligonucleotide microarray (2000 selected features), which is to date the only available oligonucleotide array for symbiotic cnidarians. We were able to identify a subset of genes clearly involved in symbiosis.
Project description:Transcriptional comparison between endodermal and ectodermal compartment in symbiotic sea anemones Anemonia viridis were analysed in several specimens. We generated an oligonucleotide microarray (2000 selected features), which is to date the only available oligonucleotide array for symbiotic cnidarians. We were able to identify a subset of genes clearly involved in symbiosis. RNA was extracted from ectodermal or endodermal samples were prepared from 4 symbiotic specimens. Hybridizations were performed against a single reference (VBl) in a dye-swap experiment.
Project description:We monitored gene expression response of the symbiotic sea anemone Anemonia viridis subjected to a thermal and/or UV stress. A symbiosis-dedicated oligonucleotide microarray (2000 selected features) was generated, representing to date the only available oligonucleotide array used for symbiotic cnidarians (GPL10546). We are describing here the expression evolution during the first phase (5 days) of the stress.
Project description:Differential expression between endodermal (zooxanthellate) and ectodermal tissue layers in the endosymbiotic sea anemone Anemonia viridis has been analyzed for 3 specimens subjected to a thermal stress (+10°C) for a 2 days period. A symbiosis-dedicated oligonucleotide microarray (2000 selected features) was generated, representing to date the only available oligonucleotide array used for symbiotic cnidarians (GPL10546). We are describing here the preferential expression in ectoderm vs endoderm (also called epidermis and gastroderm, respectively) during the time course of this thermal stress. RNA was extracted from ectodermal or endodermal dissected tissues. 3 different symbiotic anemones were subjected to a thermal and UV stress (+10°C), sampled at T0, T12h and T36h. Tissue dissections were carried out from tentacles immediately after sampling. For each sample, dye-swap hybridizations compared T0 and later time points. For each time point, statistical analysis combines the 3 biological replicates.
