Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA129385 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE21992: Analysis of HeLa cells after transfection with miR-1 or miR-155, by ribosome profiling and mRNA-Seq GSE22001: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by ribosome profiling and mRNA-Seq GSE22002: Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling GSE22003: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by microarray profiling Refer to individual Series
2010-08-02 | E-GEOD-22004 | biostudies-arrayexpress
Project description:MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output. Examine ribosome footprints and mRNA abundance of HeLa cells transfected with miR-1 or miR-155, versus mock-transfected cells, at two different time points post-transfection. Supplementary processed data files linked below. mir155_summaryTable.txt: log2 fold changes (miR-155-transfected versus mock-transfected HeLa cells, 32hr). mir1_summaryTable.txt: log2 fold changes (miR-1-transfected versus mock-transfected HeLa cells, 32hr).
2010-08-03 | E-GEOD-21992 | biostudies-arrayexpress
Project description:Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling
| PRJNA129165 | ENA
Project description:MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output. Examine mRNA expression levels in HeLa cells transfected with miR-1 or miR-155, versus mock-transfected cells, at two different time points post-transfection.
2010-08-03 | E-GEOD-22002 | biostudies-arrayexpress
Project description:We report the application of bulk RNA-Sequencing to assess the effect of miR-155 overexpression by profiling gene expression following transfection of miR-155 mimic compared against negative control mimic in human peripheral blood CD14+ monocytes. Isolated human CD14+ monocytes were transfected for 24 hours and mRNA profiles of miR-155 and negative control mimic were generated by bulk RNA-Sequencing in 5 donors. The sequence reads passed quality checks and were analysed using DeSeq2. We mapped the sequence reads to the human genome (build hg38) and identified 16,014 transcripts.
2022-02-07 | GSE166605 | GEO
Project description:RNA-seqs followed by miRNA transfections (miR-124 and miR-155) into four different cell lines( HeLa, HEK293, Huh7, and IMR90). There are two biological replicates of RNA-seqs per each miRNA transfection per each sample and there are corresponding mock transfections.
2013-12-02 | E-GEOD-52530 | biostudies-arrayexpress
Project description:Over-expression of miR-155 induces changes in the pattern of gene expression of hCMEC/D3 cells. hypothesis tested in the present study was that miR-155 constitute an important regulatory control of the brain endothelial response to inflammatory cytokines. To identify miR-155 target genes in brain endothelim that might be implicated in BBB dysfunction relevant to human disease, we then analysed changes in mRNA expression of hCMEC/D3 cells that overexpress miR-155 and results were contrasted to cells transfected with scrambled miR. To ectopically express miR-155 in hCMEC/D3 cells, 30 nM of pre-miR-155 and the siPORT Amine transfection agent (Applied Biosystems, Warrington, UK) were combined following the manufacturerM-bM-^@M-^Ys instructions.
2014-04-29 | E-GEOD-44693 | biostudies-arrayexpress
Project description:Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS.
2008-07-31 | GSE11968 | GEO
Project description:Gene expression profile following transfection with miR-503, miR-103, or miR-494 mature duplex Examination of mRNA levels in HeLa cells following transfection of miR-503, miR-103, or miR-494 mature duplex, control siRNA against GFP, or mock transfection (lipofectamine 2000 alone)
2015-02-04 | E-GEOD-64364 | biostudies-arrayexpress
Project description:To identify putative fibroblasts-specific targets of mir-155, we overexpressed mir-155 in lung fibroblasts by transfecting them with a synthetic pre-mir-155 or a synthetic “negative” pre-miRNA as control (miR-Neg). RNA samples were harvested at 24 and 48 hours post-transfection and 2 independent experiments were carried out.
2009-12-22 | GSE14477 | GEO