Project description:Genome sequencing of Legionella pneumophila subsp. pneumophila isolates from 2005 and 2008 Legionellosis outbreak in Fredrikstad, Norway
Project description:Comparaison of the transcriptional profile of mutant of the lpp1663 gene and the parent strain Legionella pneumophila strain Paris. lpp1663 codes for a protein with a ProQ domain (PFAM PF04352). A mutant of this gene was obtained by deleting the lpp1663 ORF and replacing it with a gene confering resistance to kanamycin. Transcriptional profiling was done on cultures grown to exponential phase (OD=0.8). Three independent biological replicates were analysed.
Project description:Comparaison of the transcriptional profile of mutant of the lpp0148 gene and the parent strain Legionella pneumophila strain Paris. lpp0148 codes for a protein with a ProQ domain (PFAM PF04352). A mutant of this gene was obtained by inserting a premature stop codon in the lpp0148 ORF. The lpp0148 mutant is constitutively competent for natural transformation as described here http://www.ncbi.nlm.nih.gov/pubmed/26526572. Transcriptional profiling was done on cultures grown to exponential phase (OD=0.8). Three independent biological replicates were analysed.
