Ciona intestinalis
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ABSTRACT:
PROVIDER: PRJNA131941 | ENA |
REPOSITORIES: ENA
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Project description:Among urochordates (tunicates)âthe closest living relatives of vertebratesâCiona intestinalis is increasingly being used as a model organism in the field of developmental biology. Ciona intestinalis is the seventh animal which genome published; the ~120-Mbp euchromatin region is estimated to contain ~16,000 protein-coding genes. In addition, analyses of more than one million ESTs have provided the foundation for gene models and associated transcriptomes. The fertilized Ciona intestinalis egg develops into a tadpole larva with a simplified chordate body plan, and then it metamorphose into adult sea squirt of sessile filter feeder. One of interests in the field of developmental biology is to understand what kind of genes are expressed in the body and how spatially and/or temporally coordinated expression of genes is controlled. In this study, we investigated the entire gene expression of 11 organs of adult Ciona; the neural complex, branchial sac, esophagus, stomach, endostyle, intestine, body-wall muscle, heart, blood cells, ovary, and testis. Our data would provides basic information of transcriptome in each organ and help to understand gene expression control of organ specific genes. Gene expressions in 11 organs of adult Ciona intestinalis; blood cells, branchial sac, digestive grand, endostyle, esophagus, heart, body-wall muscle, neural complex, ovary, stomach and testis. Three independent experiments were performed at each tissue using different individuals for each experiment.
2010-10-27 | E-GEOD-24941 | biostudies-arrayexpress
Project description:Among urochordates (tunicates)—the closest living relatives of vertebrates—Ciona intestinalis is increasingly being used as a model organism in the field of developmental biology. Ciona intestinalis is the seventh animal which genome published; the ~120-Mbp euchromatin region is estimated to contain ~16,000 protein-coding genes. In addition, analyses of more than one million ESTs have provided the foundation for gene models and associated transcriptomes. The fertilized Ciona intestinalis egg develops into a tadpole larva with a simplified chordate body plan, and then it metamorphose into adult sea squirt of sessile filter feeder. One of interests in the field of developmental biology is to understand what kind of genes are expressed in the body and how spatially and/or temporally coordinated expression of genes is controlled. In this study, we investigated the entire gene expression of 11 organs of adult Ciona; the neural complex, branchial sac, esophagus, stomach, endostyle, intestine, body-wall muscle, heart, blood cells, ovary, and testis. Our data would provides basic information of transcriptome in each organ and help to understand gene expression control of organ specific genes.
2010-10-27 | GSE24941 | GEO
Project description:Transcriptome Analysis of Ciona intestinalis to Identify Genes Preferentially Expressed in the Larval Brain
| PRJNA118125 | ENA
Project description:Recent whole-genome studies and in-depth expressed sequence tag (EST) analyses have identified most of the developmentally relevant genes in the urochordate, Ciona intestinalis. In this study, we made use of a large-scale oligo-DNA microarray to further investigate and identify genes with specific or correlated expression profiles. This large-scale oligo-DNA microarray for C. intestinalis should facilitate the understanding of global gene expression and gene networks during the development and ageing of a basal chordate. Keywords: gene expression, development, ageing, life cycle
2007-06-20 | GSE6308 | GEO
Project description:To compare with new Ciona intestinalis microarray NimbleGen 135k, we peformed gene expression analysis of GPL14686. Total RNAs from common samples with a part of NimbleGen's analysis are used for this analysis. Ovary and Neural Complex from adult Ciona intestinalis, duplicate, 2 time points during spawning
2011-11-05 | E-GEOD-33498 | biostudies-arrayexpress
Project description:Transcriptional profiling of Ciona intestinalis comparing ethanol exposed asidian (control) with TBT exposed ascidian.
2010-07-20 | GSE22995 | GEO
Project description:Tunicate ascidians exhibit metamorphosis that converts tadpole, swimming larva into immotile adult. In ascidian Ciona intestinalis, the mutant tail regression failed (trf) which shows defects in the metamorphosis was previously reported (Nakayama-Ishimura et al., 2009). In the metamorphosis process, trf larvae settle normally with their adhesive papillae, but do not start tail regression, papillae retraction and sensory vesicle retraction, while development of adult organs proceed. To understand the molecular mechanism of the metamorphosis, microarray analysis of trf mutant was performed.
2020-06-30 | GSE124672 | GEO
Project description:Ciona intestinalis is an invertebrate animal model system that is well characterized and has many advantages for the study of cardiovascular biology. The regulatory mechanisms of cardiac myocyte proliferation in Ciona are intriguing since Ciona are capable of regeneration throughout their lifespan. To identify important regeneration factors in Ciona, microarray analysis was conducted on RNA from adult Ciona hearts with normal or damaged myocardium using custom Affymetrix GeneChips. After a 24- or 48-hour recovery period, total RNA was isolated from damaged and control hearts. Initial results indicate significant changes in gene expression in hearts damaged by ligation in comparison to cryoinjured or control hearts. Ligation injury shows differential expression of 223 genes as compared to control (fold change >2, p<0.01, Student’s t-test) with limited false discovery (5.8%). Among these 223 genes, 117 have known human orthologs of which 68 were up-regulated and 49 were down-regulated. Notably, FGF 9/16/20 and Ras were significantly upregulated in injured hearts. Histological analyses of injured myocardium were conducted in parallel to the microarray study. Taken together, these studies will coordinate differences in gene expression to cellular changes in the regenerative myocardium of Ciona, which will help to elucidate the regulatory mechanisms of cardiac myocyte proliferation across chordates.
2024-02-06 | GSE244713 | GEO
Project description:The transcriptome of unfertilized eggs of Ciona intestinalis was determined with microarrays.
2013-10-29 | GSE45500 | GEO