Arabidopsis thaliana
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-24887
PROVIDER: PRJNA132035 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Severe loss-of-function alleles of DCL1 are embryonic lethal. Defects in cell division were seen as early as the globular stage in the strong loss-of-function allele dcl1-15. Phenotypic work with dcl1-15 and the null allele dcl1-5 suggested that, in addition to the severe patterning defects, the mutants were maturing earlier than wild-type embryos. We performed global gene expression analysis of dcl1-15 and wild-type torpedo staged embryos to examine this maturation phenotype further. The results suggested that DCL1 is a heterochronic gene. Comparisons to a time series of embryo development (http://www.seedgenenetwork.net/arabidopsis) showed that the genes differentially expressed in dcl1-15 embryos behaved more like green-cotyledon stage embryos than torpedo embryos.
2011-02-17 | GSE24887 | GEO
Project description:Severe loss-of-function alleles of DCL1 are embryonic lethal. Defects in cell division were seen as early as the globular stage in the strong loss-of-function allele dcl1-15. Phenotypic work with dcl1-15 and the null allele dcl1-5 suggested that, in addition to the severe patterning defects, the mutants were maturing earlier than wild-type embryos. We performed global gene expression analysis of dcl1-15 and wild-type torpedo staged embryos to examine this maturation phenotype further. The results suggested that DCL1 is a heterochronic gene. Comparisons to a time series of embryo development (http://www.seedgenenetwork.net/arabidopsis) showed that the genes differentially expressed in dcl1-15 embryos behaved more like green-cotyledon stage embryos than torpedo embryos. Seeds of a DCL1/dcl1-15 plant were sown. For each wild-type sample, 300 torpedo stage embryos were selected from wild-type siblings. For each mutant replicate, 300 dcl1-15/dcl1-15 embryos were selected from the siliques of DCL1/dcl1-15 where the wild-type embryos were at the torpedo stage.
2011-02-17 | E-GEOD-24887 | biostudies-arrayexpress
Project description:Transcription profiling by array of Arabidopsis wild-type and dcl1-15 torpedo-staged embryos
2014-10-10 | E-GEOD-24887 | ExpressionAtlas
Project description:Transcript profiling of wild-type and dcl1-5 early globular embryos
| PRJNA134143 | ENA
Project description:Expression data from torpedo-stage embryos from different Arabidopsis accessions
| PRJNA208237 | ENA
Project description:DICER-LIKE1 (DCL1) is required for miRNA biogenesis and embryonic pattern formation. Presumably the patterning defects observed in dcl1-5 null embryos are due to the loss of miRNAs and the consequent up-regulation of their respective targets. To test which miRNA targets were up-regulated in dcl1 embryos relative to wild-type embryos, we performed genome-wide transcript profiling of wild-type and dcl1-5 early globular embryos using directional mRNA-Seq.
2010-12-01 | GSE25404 | GEO
Project description:adt05-04_drn - mutant vs wildtype - DRN targets - Ovules containing heart-stage/torpedo-stage embryos were dissected from siliques and imidiately shock frozen in liquid nitrogen. RNA was extracted and sent to Evry for comparison between mutant and wildtype transcriptome. Keywords: gene knock out
2010-05-01 | GSE19800 | GEO
Project description:Expression data from Arabidopsis C3H14/15 overexpressors, c3h14c3h15 double mutant vs wild type
| PRJNA232445 | ENA
Project description:adt05-04_drn - mutant vs wildtype - DRN targets - Ovules containing heart-stage/torpedo-stage embryos were dissected from siliques and imidiately shock frozen in liquid nitrogen. RNA was extracted and sent to Evry for comparison between mutant and wildtype transcriptome. Keywords: gene knock out 2 dye-swap - CATMA arrays
2010-04-30 | E-GEOD-19800 | biostudies-arrayexpress
Project description:Expression data from aerial parts of 14-day-old wild-type and GFP-TIE1-15 Arabidopsis seedlings
| PRJNA167546 | ENA