Project description:The leaf of Eriobotrya japonica (Ej) has been used for a long time as an oriental medicine to treat pulmonary inflammatory diseases. This study investigated the gene expression changes by lipopolysaccharide (LPS) stimulation in the cultured human gingival fibroblast and the gene expression changes by the leaves of Ej when challenged with LPS using a microarray chip. Human gingival fibroblast were divided into three experimental groups; â C: Control, â¡ LPS: LPS-treatment only, ⢠LPS-Ej: LPS- and Ej-treatments. Total RNA was isolated from each experimental fibroblast (3 experimental group à 1 sample of each experimental group = total 3 samples).
Project description:The leaf of Eriobotrya japonica (Ej) has been used for a long time as an oriental medicine to treat pulmonary inflammatory diseases. This study investigated the gene expression changes by lipopolysaccharide (LPS) stimulation in the cultured human gingival fibroblast and the gene expression changes by the leaves of Ej when challenged with LPS using a microarray chip.
Project description:The Moutan Cortex Radicis (MCR) has been used as an analgesic, sedative and anti-inflammatory agent. This study investigated the changes in gene expression by MCR treatment when stimulated with lipopolysaccharide (LPS) in cultured human gingival fibroblasts (HGFs) and the gene expression changes by the MCR when challenged with LPS using a microarray chip. Human gingival fibroblast were divided into three experimental groups; 1, C: Control, 2, LPS: LPS-treatment only, 3, MCR40: LPS- and MCR40-treatments. Total RNA was isolated from each experimental fibroblast (3 experimental group M-CM-^W 1 sample of each experimental group = total 3 samples).
Project description:The Artemisia iwayomogi (Ai) has been known to inhibit the inflammatory cytokine production and the allergic reactions, and has been used to treat liver diseases. This study investigated the gene expression changes by lipopolysaccharide (LPS) stimulation in the cultured human gingival fibroblast and the gene expression changes by the Ai when challenged with LPS using a microarray chip. Human gingival fibroblast were divided into three experimental groups; â C: Control, â¡ LPS: LPS-treatment only, ⢠LPS-Ai: LPS- and Ai-treatments. Total RNA was isolated from each experimental fibroblast (3 experimental group à 1 sample of each experimental group = total 3 samples).
Project description:The Artemisia iwayomogi (Ai) has been known to inhibit the inflammatory cytokine production and the allergic reactions, and has been used to treat liver diseases. This study investigated the gene expression changes by lipopolysaccharide (LPS) stimulation in the cultured human gingival fibroblast and the gene expression changes by the Ai when challenged with LPS using a microarray chip.
