Project description:Effect of the high nitrogen insensitive 9 (hni9) mutation in contrasted nitrogen environments. hni9 vs. WT, and Low Nitrogen vs. High Nitrogen environments. 2 biological replicates per genotype/environment.
Project description:adt05-04_drn - mutant vs wildtype - DRN targets - Ovules containing heart-stage/torpedo-stage embryos were dissected from siliques and imidiately shock frozen in liquid nitrogen. RNA was extracted and sent to Evry for comparison between mutant and wildtype transcriptome. Keywords: gene knock out
Project description:Nitrogen and light are two major regulators of plant metabolism and development. While genes involved in the control of each of these signals have begun to be identified, regulators that integrate gene responses to nitrogen and light signals have yet to be determined. Here, we evaluate the role of bZIP1, a transcription factor involved in light and nitrogen sensing, by exposing wild-type (WT) and bZIP1 T-DNA null mutant plants to a combinatorial space of N and L treatment conditions. We use ANOVA analysis combined with clustering and Boolean modeling, to evaluate the role of bZIP1 in mediating L and N signaling genome-wide.
Project description:adt05-04_drn - mutant vs wildtype - DRN targets - Ovules containing heart-stage/torpedo-stage embryos were dissected from siliques and imidiately shock frozen in liquid nitrogen. RNA was extracted and sent to Evry for comparison between mutant and wildtype transcriptome. Keywords: gene knock out 2 dye-swap - CATMA arrays
