Project description:Examination of gene expression pattern in Triticum monococcum line KU-104-1 and its mvp2 mutant during the vegetative/generative transition at cold temperature conditions.
Project description:Wheat grain storage proteins (GSPs) make up most of the protein content of grain and determine flour end-use value. The synthesis and accumulation of GSPs depend highly on nitrogen (N) and sulfur (S) availability and it is important to understand the underlying control mechanisms. Here we studied how the einkorn (Triticum monococcum ssp. monococcum) grain proteome responds to different amounts of N and S supply during grain development. Two subproteomes were analyzed : albumin-globulin and nuclear proteins.
Project description:24-hour cold shock treatment applied to winter wheat T. monococcum ssp. aegilopoides (accession G3116) and the domesticated spring wheat T. monococcum ssp. monococcum (accession DV92). Tissues were harvested and homogenized using liquid nitrogen. RNA extraction was conducted using a modified chloroform extraction protocol (Plant Purification Reagent) followed by RNeasy Plant Mini Kit (50) for purification. Samples included: three G3116 control, three G3116 cold shock, three DV92 control, and three DV92 cold shock. Illumina samples were prepped using TruSeq RNA prep kit v.2 and sequenced on the Illumina Hiseq 2500 sequencing platform.
