Project description:We report the application of Cappable-seq to selectively enrich prokaryotic endosymbiont transcripts from mixed host-symbiont total RNA.

2017-01-05 | GSE93139 | GEO

Identification of cross-species microRNA-based Communication in Brugia malayi with its endosymbiont Wolbachia

Project description:Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. A total of 178 novel microRNA were identified from short read transcriptional data, which when combined with known Brugia microRNAs yielded a total of 284 microRNA. Of these, 123 microRNA sequences (43%) are differentially expressed over the mammalian life stages of B. malayi that we examined. Putative targets of these microRNA were identified from inversely expressed target clusters that contain valid seed sequences for the corresponding microRNAs. The largest identified cluster is downregulated in adult females and enriched in zinc finger domains, helicase domains, and DNA binding domains suggesting this microRNA cluster may have regulatory control over a large proportion of adult female specific mRNA genes. MicroRNA-like molecules are identified as produced by the Wolbachia endosymbiont, providing evidence for direct nucleic acid-based interdomain communication between filarial nematodes and their bacterial obligate endosymbiont.

2022-01-15 | GSE143730 | GEO

Tissue-Specific Transcriptomics and Proteomics of a Filarial Nematode and its Wolbachia Endosymbiont

Project description:We sequenced total RNA from Dirofilaria immitis in order to generate the first tissue-specific gene expression profile of a filarial nematode and its Wolbachia endosymbiont.

2016-06-01 | GSE67894 | GEO

Wolbachia endosymbiont of Drosophila willistoni

Project description:Wolbachia endosymbiont of Drosophila willistoni overview

| PRJNA35895 | ENA

Wolbachia endosymbiont of Drosophila ananassae

Project description:Wolbachia endosymbiont of Drosophila ananassae overview

| PRJNA35465 | ENA