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Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity

ABSTRACT: Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity

PROVIDER: PRJNA134139 | ENA |

REPOSITORIES: ENA

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Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity

Project description:This SuperSeries is composed of the following subset Series: GSE25401: Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [gene expression] GSE25470: Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [miRNA data] GSE25910: Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [differentiation data] Refer to individual Series

2012-08-12 | E-GEOD-25402 | biostudies-arrayexpress

Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [miRNA data]

2012-08-13 | GSE25470 | GEO

Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [gene expression]

2012-08-13 | GSE25401 | GEO

Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity (differentiation data)

2012-08-13 | GSE25910 | GEO

Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [gene expression]

Project description:We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity. Abdominal subcutaneous adipose needle biopsies were obtained from women (n=56) with a wide variation in BMI. From the biopsies we prepared and hybridised biotinylated complementary RNA to GeneChip Human Gene 1.0 ST Arrays (Affymetrix Inc., Santa Clara, CA), labelled RNA to Affymetrix miRNA arrays, and bisulphite converted DNA to Illumina Infinium HumanMethylation27 beadchips using standardised protocols (Affymetrix Inc., Illumina Inc.) . Subsequent analyses of gene expression was performed using the Affymetrix GeneChip Operating Software version 1.4. To allow comparisons of transcript levels between samples, all samples were subjected to an all-probeset scaling-to-target signal of 100.

2012-08-12 | E-GEOD-25401 | biostudies-arrayexpress

Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [miRNA data]

Project description:We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity. Abdominal subcutaneous adipose needle biopsies were obtained from women (n=56) with a wide variation in BMI. From the biopsies, total RNA was isolated and labelled using the FlashTag biotin HSR labeling kit (Genisphere Inc., Hatfield, PA) according to the supplier's protocol. The labelled samples were placed in a hybridization cocktail mix containing 4% formamide and hybridised overnight to Affymetrix miRNA Arrays (Affymetrix Inc.) following the indicated Genisphere protocol. The arrays were washed, stained and scanned in an Affymetrix GCS 3000 scanner. Signal intensities and present calls were generated by using the microRNA QC tool by Affymetrix.

2012-08-12 | E-GEOD-25470 | biostudies-arrayexpress

Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity (differentiation data)

Project description:We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity. Subcutaneous adipose tissue was obtained from healthy subjects undergoing cosmetic liposuction (n=12). There was no selection for age, sex or BMI. From the tissue, we isolated preadipocytes and in vitro differentiated them to adipocytes. The cells were lysed at day 4/5 (early), 8 (middle) and 12 (late) of differentiation. From the samples, we prepared and hybridised biotinylated complementary RNA to GeneChip Human Gene 1.0 ST Arrays (Affymetrix Inc., Santa Clara, CA). Pre-processing was performed using the Affymetrix Expression Console version 1.1 and the following settings: Summarization: PLIER; Background Correction: PM-GCBG; Normalization: Global Median.

2012-08-12 | E-GEOD-25910 | biostudies-arrayexpress

Adipose tissue microRNAs as regulators of CCL2 production in human obesity.

Project description: Not available

| S-EPMC3402332 | biostudies-literature

Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-08-13 | GSE25402 | GEO

The Ubiquitin Ligase Siah2 Regulates Obesity-induced Adipose Tissue Inflammation

Project description:Chronic, low-grade adipose tissue inflammation associated with adipocyte hypertrophy is an important link in the relationship between obesity and insulin resistance. Although ubiquitin ligases are essential regulators of inflammatory processes, the role of these enzymes in metabolically driven adipose tissue inflammation is relatively unexplored. In this study, we found that the ubiquitin ligase Siah2 is a central factor in obesity-related adipose tissue inflammation. When challenged with chronic excess energy intake, Siah2-null mice become obese with enlarged adipocytes, but do not develop obesity-induced insulin resistance. Proinflammatory gene expression is substantially reduced in the Siah2-null epididymal adipose tissue of the obese Siah2KO mice.

2015-09-29 | GSE61839 | GEO

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