Project description:The goal of this study is to identify ERalpha-target genes affected by knocking down of the histone arginine methyltransferase CARM1 in MCF7 breast cancer cells. The roles of CARM1 in ERalpha+ breast cancer was not well characterized. Therefore, we created a Dox inducible CARM1 knockingdown MCF7 cell line where CARM1 is decreased to 20% of endogeneous level to determine the created a Dox-inducible CARM1shRNA overexpressing MCF7 cells for evaluation of the global effects of CARM1 on ERalpha-target gene expression. MCF7-tet-on-CARM1shRNA clone 1 were treated under 4 conditions: DMSO; Dox; E2 (10nM); Dox+E2. In Dox+E2 condition, cells were pre-treated with Dox for 5 days before treating with E2 for 4 hours. 3 biological replicates were included and total of 12 samples were analyzed.

2011-06-01 | E-GEOD-26259 | biostudies-arrayexpress

Homo sapiens

Project description:Gene Expression Profiling of SPOP Knocked Down Cell

| PRJNA392902 | ENA

Homo sapiens

Project description:Expression profiling of RBM38-knocked down and control MCF7 cells with and without doxorubicin treatment

| PRJNA146375 | ENA

Homo sapiens

Project description:Gene expression profile in MCF7 breast cancer cells after 78 functionallly important molecules were knocked down using siRNA.

| PRJNA145053 | ENA

Gene expression analysis in Eight Homo sapiens Cell lines

Project description:Gene expression profiles for ~20,000 genes using Illumina Homo sapiensRef-8 v2