Project description:This SuperSeries is composed of the following subset Series: GSE25273: Array Comparative Genomic Hybridization of Pancreatic Xenografts and Cell Lines GSE26088: Expression Profiling of Pancreatic Xenografts and Cell Lines Refer to individual Series
Project description:Array comparative genomic hybridization (aCGH) analysis of chromosome 3 in human mesothelioma cells grown in cell culture or as xenografts
Project description:We compared the genomic integrity of human P493 cells grown in vitro with those recovered from xenografts of P493 cells. Keywords: single nucleotide polymorphism, array comparative genomic hybridization
Project description:Array Comparative Genomic Hybridization (aCGH) of 70 pancreatic ductal adenocarcinoma (PDAC) samples was performed on Agilent 244K CGH arrays in order to find common genomic aberrations for cancer gene discovery. Additionally, matched expression profiling on Agilent 44K arrays was performed. Common copy number aberrations were identified in order to identify a list of putative cancer genes. Expression profiling data was used to further enrich this list of putative cancer genes for more likely candidates. Last, the most promising candidates were functionally interrogated using RNA interference-mediated knockdown to mimic loss. Well-known PDAC cancer genes were observed as amplified (KRAS and MYC) and deleted (CDKN2A, TGFBR2, SMAD4, and MAP2K4). 70 tumor samples (48 xenografts, 22 cell lines). 2-color arrays hybridized against a common reference pool of genomic DNA from 8 normal individuals. This dataset represents the aCGH component of the study.
Project description:To directly compare genomic alterations between patient tumors and derived cell lines, both parental tumors and cell lines were characterized by array-based comparative genomic hybridization.
Project description:Primary cell cultures were isolated from different KrasG12D-driven mouse models of pancreatic cancers and subjected to array comparative genomic hybridization (aCGH) for the investigation of copy number profiles.
