Project description:Here we developed a new high-throughput polymorphism detection and genotyping method based on identifying restriction cut site polymorphisms using a microarray platform. We compared the genomes of 20 individual urchins; 10 from the northern part of the species range (Boiler Bay, OR) and 10 from the southern part of the range (San Diego, CA).
Project description:Environmental extracts and fractions obtained with passive sampling (HP-20, SMIRC) from Mission Bay, Point Loma, Scripps Pier, Salton Sea (San Diego and Imperial Counties)
Project description:Genomic DNA extracted from two different Photobacterium profundum strains: SS9 strain (completely sequenced and used to made the microarray) and 3TCK strain were labeled with Cy3 and Cy5 fluorophores and competitively hybridized on the microarray built on the basis of the SS9 strain genomic sequence. Aim: the identification of the genomic regions absent in the 3TCK strain with respect to the SS9 strain. The SS9 strain was isolated from the Sulu Trench and displays an optimum growth at 28 MPa (2800 metres of depth). The 3TCK strain was isolated from a coastal sample collected near San Diego, California and has an optimum growth at 0.1 MPa.
