Project description:We used massively parallel sequencing to discover and characterize small RNAs (sRNAs) from fission yeast Schizosaccharomyces japonicus. We found that, unlike in related S. pombe, a substantial fraction of sRNAs maps to transposons, both telomeric and centromeric. small RNA library from total RNA isolations from Schizosaccharomyces japonicus
Project description:We used massively parallel sequencing to discover and characterize small RNAs (sRNAs) from fission yeast Schizosaccharomyces japonicus. We found that, unlike in related S. pombe, a substantial fraction of sRNAs maps to transposons, both telomeric and centromeric.
Project description:small RNA-seq was performed to identify differences in small RNA complement between wild type and Dcr1 deficent cells in Schizosaccharomyces japonicus.
Project description:Small interfering RNAs (siRNAs) are known to be involved in both transposon silencing and centromere function, leading us to investigate the interplay between these two roles in the Schizosaccharomyces lineage. In S. pombe, the centromeric repeats produce dicer-dependent siRNAs that are required for maintenance of centromeric structure, function and transcriptional silencing via Argonaute-dependent heterochromatin formation13. However, transposons are silenced in S. pombe by RNAi-independent mechanisms and do not produce abundant siRNAs. To investigate whether centromere-directed siRNA production is conserved within the transposon-rich centromeres of S. japonicus, we isolated and sequenced small RNAs from log-phase S. japonicus cultures. The small RNAs have a modal size of 23 nucleotides and 94% map to transposons, both telomeric and centromeric.
Project description:Small interfering RNAs (siRNAs) are known to be involved in both transposon silencing and centromere function, leading us to investigate the interplay between these two roles in the Schizosaccharomyces lineage. In S. pombe, the centromeric repeats produce dicer-dependent siRNAs that are required for maintenance of centromeric structure, function and transcriptional silencing via Argonaute-dependent heterochromatin formation13. However, transposons are silenced in S. pombe by RNAi-independent mechanisms and do not produce abundant siRNAs. To investigate whether centromere-directed siRNA production is conserved within the transposon-rich centromeres of S. japonicus, we isolated and sequenced small RNAs from log-phase S. japonicus cultures. The small RNAs have a modal size of 23 nucleotides and 94% map to transposons, both telomeric and centromeric. Isolation and computational analysis of small RNAs from wild-type S. japonicus
Project description:ChIP-seq was performed to identify differences in H3K9me2 profiles between wild type and Dcr1 deficent cells in Schizosaccharomyces japonicus.
Project description:We have identified that these two organisms have quite distinct phospholipid structures - S. pombe phospholipids display canonical symmetric long acyl chains (18 and/ or 16 carbon atoms), and in S. japonicus significant fraction of phospholipids are asymmetrical - one acyl chain is 18 carbon atoms and second one is short - only 10 carbon atoms. Medium chain fatty acid like C10:0 are critical for S. japonicus physiology and are synthesised by the cytosolic fatty acid synthase (FAS) complex. Such findings lead us to address the functional role of these asymmetric lipids and we exchanged FAS between the two fission yeast species. We observed that in S. pombe expressing solely japonicus FAS, the membrane is functionally impaired in a number of ways (e.g. endocytosis, mitosis etc etc) and moreover, it seems that at lower temperatures (such as 24C) the phenotype is more severe compared to the higher temperatures like 30 and 36. We believe that comparative transcriptomic analysis will shed light on what is going on in S. pombe cells with exchanged FAS and therefore displaying an altered lipidome.
