Project description:Gene expression of characteristic chondrogenic markers and miRNA expression were analyzed in cells cultured in differentiation medium and significant differences were found between gelation/PRP microgels and those containing only pure gelatin. We used microarrays to detail the miRNA expression in studied cell cultures for identification the expression of miRNA and study the up- and down-regulated miRNA associated.
Project description:These data show time-course transcriptional profiling of porcine subcutaneous preadipocyte cell line (PSPA) during differentiation. In the early stage after stimulation for adipocyte differentiation, upregulation of many genes encoding proteins involved in regulation of cell proliferation and transcription was observed. The data presented here should form a valuable database for exploring candidate genes for fat-related QTLs in pigs, and also for analyses to clarify the physiological characteristics of development of porcine adipose tissues by comparison with the results in humans and mice.